Shalitin C, Fischer I
Biochim Biophys Acta. 1975 Dec 19;414(3):263-72. doi: 10.1016/0005-2787(75)90165-3.
Hybridization experiments using uniformly labeled poly(A) RNA derived from Saccharomyces cerevisiae strains carrying the "killer character" showed that (1) these molecules appear to be transcribed from repetitive DNA sequences. (2) there are approximately 35 DNA template sequences that are transcribed into poly(A) RNA. It is concluded that under the RNA extraction procedure used, most of the poly (A) RNA represents killer-RNA as judged by the dependence of the kinetic complexity of poly(A) RNA on the genomic complexity of killer-RNA.
使用从携带“杀伤特性”的酿酒酵母菌株中提取的均匀标记的聚腺苷酸RNA进行的杂交实验表明:(1)这些分子似乎是从重复DNA序列转录而来的。(2)大约有35个DNA模板序列被转录成聚腺苷酸RNA。得出的结论是,在所使用的RNA提取程序下,根据聚腺苷酸RNA的动力学复杂性对杀伤RNA基因组复杂性的依赖性判断,大多数聚腺苷酸RNA代表杀伤RNA。