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酿酒酵母核糖核酸中聚腺苷酸的结构

Structure of polyadenylic acid in the ribonucleic acid of Saccharomyces cerevisiae.

作者信息

Phillips S L, Tse C, Serventi I, Hynes N

出版信息

J Bacteriol. 1979 May;138(2):542-51. doi: 10.1128/jb.138.2.542-551.1979.

Abstract

Investigations of the structure of polyadenylic acid [poly(A)] in yeast have shown that there are two classes of poly(A) distinguished by size and kinetics of synthesis. Each class is found directly on the 3' end of messenger RNA. One class contains poly(A) molecules ranging from 60 to less than 20 nucleotides long. The longest molecules in this poly(A) class are the first to become labeled when cells are exposed to [3H]adenine. Label then appears in progressively smaller molecules. The second class of poly(A) is about 20 nucleotides long. The length homogeneity of this class and the presence in nuclear DNA of many copies of a polythymidylate sequence which is the same length suggests that this poly(A) is synthesized by transcription from DNA.

摘要

对酵母中聚腺苷酸[poly(A)]结构的研究表明,有两类聚(A)可通过合成的大小和动力学来区分。每一类都直接位于信使RNA的3'末端。一类含有长度从60到少于20个核苷酸的聚(A)分子。当细胞暴露于[3H]腺嘌呤时,这类聚(A)中最长的分子最先被标记。随后标记出现在逐渐变小的分子中。第二类聚(A)约20个核苷酸长。这类聚(A)的长度均匀性以及核DNA中存在许多相同长度的聚胸腺嘧啶序列拷贝,表明这类聚(A)是由DNA转录合成的。

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Microbiol Rev. 1993 Jun;57(2):383-401. doi: 10.1128/mr.57.2.383-401.1993.

本文引用的文献

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Macromolecule synthesis in yeast spheroplasts.酵母原生质体中的大分子合成。
J Bacteriol. 1967 Nov;94(5):1697-705. doi: 10.1128/jb.94.5.1697-1705.1967.
4
The determination of base ratios of very small samples of ribonucleic acid using thin layer chromatography.
J Chromatogr. 1968 Apr 23;34(3):364-9. doi: 10.1016/0021-9673(68)80068-8.
6
The separation of mouse reticulocyte 9S RNA into fractions of different biological activity by hybridization to poly(U)-cellulose.
Biochem Biophys Res Commun. 1972 Nov 1;49(3):775-81. doi: 10.1016/0006-291x(72)90478-0.

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