Suzuki I, Martin S, Boursalian T E, Beers C, Fink P J
Department of Immunology, University of Washington, Seattle, WA 98195, USA.
J Immunol. 2000 Nov 15;165(10):5537-43. doi: 10.4049/jimmunol.165.10.5537.
Fas ligand (FasL/CD95L/APO-1L) is one of a growing number of TNF family members whose triggering costimulates maximal proliferation of activated T cells. In this study we show that maximal Ag-dependent accumulation of transferred TCR-transgenic CD8(+) T cells requires Fas (CD95/APO-1) expression by the adoptive hosts. Additionally, adoptively transferred FasL(+) CD8(+) T cells demonstrate a 2-fold advantage in Ag-driven expansion over their FasL(-)counterparts. This study illustrates the in vivo role of TCR-dependent FasL costimulation in the Ag-specific proliferation of both heterogeneous and homogeneous populations of primary CD8(+) T cells and long-term CTL lines. Thus, cross-linking FasL on naive and Ag-experienced CD8(+) T cells whose Ag-specific TCRs are engaged is required to drive maximal cellular proliferation in vivo.
Fas配体（FasL/CD95L/APO-1L）是肿瘤坏死因子（TNF）家族中越来越多的成员之一，其触发可共刺激活化T细胞的最大增殖。在本研究中，我们发现，过继转移的TCR转基因CD8(+) T细胞在抗原依赖下的最大积累需要过继宿主表达Fas（CD95/APO-1）。此外，过继转移的FasL(+) CD8(+) T细胞在抗原驱动的扩增中比其FasL(-)对应细胞表现出2倍的优势。本研究阐明了TCR依赖性FasL共刺激在原代CD8(+) T细胞的异质性和同质性群体以及长期CTL系的抗原特异性增殖中的体内作用。因此，对于抗原特异性TCR被激活的幼稚和经历过抗原刺激的CD8(+) T细胞，交联FasL是体内驱动最大细胞增殖所必需的。
