Gesase A P, Kiyama H
Department of Anatomy/Histology, Muhimbili University College of Health Sciences, Dar-Es-salaam, Tanzania.
J Neurocytol. 2000 Jan;29(1):61-6. doi: 10.1023/a:1007116214485.
Following hypoglossal nerve transection, the microglia of the rat hypoglossal nucleus expressed protein kinase CK2 beta subunit immunoreactivity. CK2 beta immunostaining occurred on the operated side from postoperative day 3; on day 5 we observed strong immunoreactivity and the immunopositive microglial cell processes surrounded the injured neurones. Thereafter, the immunoreactivity decreased gradually and on day 10 the immunopositive cells surrounded only a few injured neurones. Electron microscopic observations on the hypoglossal nucleus revealed microglia-neuronal contact within 3 hours of nerve injury, and by day 3 all the injured neurones were in contact with microglial cells. These observations indicated that microglia-neuronal contact occurred earlier than the CK2 beta subunit immunoreactivity. CK2 may not be implicated during the initial migration of the microglia to the injured neurones; however, it may enhance the growth and elongation of the microglial cell processes around the injured neurones.
舌下神经横断后，大鼠舌下神经核的小胶质细胞表达蛋白激酶CK2β亚基免疫反应性。CK2β免疫染色从术后第3天开始出现在手术侧；在第5天，我们观察到强烈的免疫反应性，免疫阳性的小胶质细胞突起围绕着受损神经元。此后，免疫反应性逐渐降低，在第10天，免疫阳性细胞仅围绕少数受损神经元。对舌下神经核的电子显微镜观察显示，在神经损伤后3小时内小胶质细胞与神经元发生接触，到第3天，所有受损神经元都与小胶质细胞接触。这些观察结果表明，小胶质细胞与神经元的接触比CK2β亚基免疫反应性出现得更早。CK2可能在小胶质细胞向受损神经元的初始迁移过程中未起作用；然而，它可能会增强受损神经元周围小胶质细胞突起的生长和伸长。
