Svensson M, Aldskogius H
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Eur J Neurosci. 1993 Jan 1;5(1):85-94. doi: 10.1111/j.1460-9568.1993.tb00208.x.
The aim of the present study was to examine whether inhibition of the microglial cell reaction around axotomized motoneurons affects the subsequent regeneration process of the injured axons. The microglial cell reaction in the hypoglossal nucleus of the rat was blocked by infusion of cytosine-arabinoside (ARA-C) into the ventricular system. Axon regeneration was evaluated by determining the number and size distribution of myelinated axons at a defined level distal to the crush site, the number of neurons which could be retrogradely labelled from the distal stump as well as the number of motor endplates in the tongue at various times following injury. No significant difference was observed for any of these parameters between ARA-C-treated and untreated animals. Therefore, it is concluded that the microglial cell reaction is not necessary for peripheral nerves to regenerate and restore target contact at a normal rate and to a normal extent.
本研究的目的是检验对轴突切断的运动神经元周围小胶质细胞反应的抑制是否会影响受损轴突随后的再生过程。通过向脑室系统注入阿糖胞苷（ARA-C）来阻断大鼠舌下神经核中的小胶质细胞反应。通过确定损伤部位远端特定水平处有髓轴突的数量和大小分布、可从远端残端逆行标记的神经元数量以及损伤后不同时间舌部运动终板的数量来评估轴突再生。在接受ARA-C处理和未处理的动物之间，这些参数均未观察到显著差异。因此，得出结论，小胶质细胞反应对于外周神经以正常速度和正常程度再生并恢复与靶标的接触并非必需。
