Boujtita M, El Murr N
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes (CNRS UPRSA 6006), Faculté des Sciences et des Techniques, Nantes, France.
Appl Biochem Biotechnol. 2000 Oct;89(1):55-66. doi: 10.1385/abab:89:1:55.
A mediated modified carbon paste and renewable surface electrode for fructose amperometric measurement based on D-fructose dehydrogenase (FDH) was prepared and optimized. Commercially available ferrocene (FcH) and hydroxymethyl ferrocene (FcCH2OH) were used as mediators. The substituted FcH showed better linearity and higher sensitivity. The influence of different experimental parameters was studied for optimum analytical performance. The final FDH-modified electrode showed good analytical performance for batch mode measurements of fructose.
制备并优化了一种基于D-果糖脱氢酶（FDH）的用于果糖安培测量的介导修饰碳糊和可再生表面电极。使用市售的二茂铁（FcH）和羟甲基二茂铁（FcCH2OH）作为介导剂。取代的FcH表现出更好的线性和更高的灵敏度。研究了不同实验参数的影响以获得最佳分析性能。最终的FDH修饰电极在果糖的批量模式测量中表现出良好的分析性能。
