用于通过流动注射分析测定果汁中葡萄糖和蔗糖的生物传感器。

Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis.

作者信息

Guémas Y, Boujtita M, el Murr N

机构信息

Groupe Electrochimie-Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes (UPRSA-CNRS 6006), Faculté des Sciences et des Techniques, 2, rue de la Houssinière, B.P. 92208 44322 Nantes, France.

出版信息

Appl Biochem Biotechnol. 2000 Nov-Dec;89(2-3):171-81. doi: 10.1385/abab:89:2-3:171.

DOI:10.1385/abab:89:2-3:171

PMID:11209461

Abstract

Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with < 3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruit juices. The results were also compared with those obtained with the ultraviolet method.

摘要

采用流动注射分析技术，通过安培法测定葡萄糖和蔗糖。使用含有葡萄糖氧化酶、辣根过氧化物酶和二茂铁的具有可再生表面的碳糊电极，并结合可溶性酶转化酶和变旋酶。研究了转化酶、变旋酶和抗坏血酸对电极响应的影响。测定葡萄糖和蔗糖浓度的误差小于3%。所提出的葡萄糖和蔗糖测量方法在果汁实际样品中得到验证。还将结果与用紫外法获得的结果进行了比较。