Ege University, Faculty of Science, Biochemistry Department 35100 Bornova, Izmir, Turkiye.
Talanta. 2011 Dec 15;87:67-73. doi: 10.1016/j.talanta.2011.09.042. Epub 2011 Sep 29.
An electrochemical biosensor for detection of fructose in food samples was developed by immobilization of fructose dehydrogenase (FDH) on cysteamine and poly(amidoamine) dendrimers (PAMAM)-modified gold electrode surface. Electrochemical analysis was carried out by using hexacyanoferrate (HCF) as a mediator and the response time was 35s at +300 mV vs. Ag/AgCl. Moreover, some parameters such as pH, enzyme loading and type of PAMAM (Generations 2, 3 and 4) were investigated. Then, the FDH biosensor was calibrated for fructose in the concentration range of 0.25-5.0mM. To evaluate its utility, the FDH biosensor was applied for fructose analysis in real samples. Finally, obtained data were compared with those measured with HPLC as a reference method.
一种电化学生物传感器，用于检测食品样品中的果糖，通过将果糖脱氢酶（FDH）固定在半胱胺和聚（酰胺-胺）树枝状聚合物（PAMAM）修饰的金电极表面上制备而成。电化学分析采用铁氰化钾（HCF）作为媒介，在+300 mV 相对于 Ag/AgCl 的情况下，响应时间为 35s。此外，还研究了一些参数，如 pH 值、酶载量和 PAMAM 的类型（第二代、第三代和第四代）。然后，FDH 生物传感器在 0.25-5.0mM 的果糖浓度范围内进行了校准。为了评估其实用性，将 FDH 生物传感器应用于实际样品中的果糖分析。最后，将获得的数据与 HPLC 作为参考方法测量的数据进行了比较。
