Saeki H, Wu M T, Olasz E, Hwang S T
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892-5370, USA.
Eur J Immunol. 2000 Oct;30(10):2808-14. doi: 10.1002/1521-4141(200010)30:10<2808::AID-IMMU2808>3.0.CO;2-K.
Chemokine receptors on dendritic cells (DC) and chemokines within lymph nodes (LN) contribute to trafficking of DC to appropriate sites within the LN. Here we show that DC that have migrated out of skin ex vivo (migratory DC, migDC) express 50-fold more CXCR5 mRNA than fresh Langerhans cells and migrate in response to B lymphocyte chemoattractant (BLC) in vitro. When injected into the footpad of mice, migDC emigrate to regional LN where up to 40% are found in B cell zones. By contrast, murine bone marrow-derived DC display 14-fold less CXCR5, do not migrate to BLC in vitro, and migrate strictly to T cell zones in LN. We propose that activated skin DC utilize CXCR5 and BLC as a possible mechanism to home to B cell zones of LN, where they may have direct effects on B cells.
树突状细胞（DC）上的趋化因子受体以及淋巴结（LN）内的趋化因子，有助于DC迁移至LN内的适当位置。在此我们表明，离体后从皮肤迁出的DC（迁移性DC，migDC）表达的CXCR5 mRNA比新鲜朗格汉斯细胞多50倍，并且在体外对B淋巴细胞趋化因子（BLC）产生迁移反应。当注射到小鼠足垫中时，migDC迁移至局部LN，其中高达40%存在于B细胞区。相比之下，小鼠骨髓来源的DC表达的CXCR5少14倍，在体外不向BLC迁移，并且严格迁移至LN的T细胞区。我们提出，活化的皮肤DC利用CXCR5和BLC作为归巢至LN的B细胞区的一种可能机制，在该区域它们可能对B细胞产生直接影响。
