Ssn6-Tup1与抑制所需的I类组蛋白去乙酰化酶相互作用。
Ssn6-Tup1 interacts with class I histone deacetylases required for repression.
作者信息
Watson A D, Edmondson D G, Bone J R, Mukai Y, Yu Y, Du W, Stillman D J, Roth S Y
机构信息
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
出版信息
Genes Dev. 2000 Nov 1;14(21):2737-44. doi: 10.1101/gad.829100.
Abstract
Ssn6-Tup1 regulates multiple genes in yeast, providing a paradigm for corepressor functions. Tup1 interacts directly with histones H3 and H4, and mutation of these histones synergistically compromises Ssn6-Tup1-mediated repression. In vitro, Tup1 interacts preferentially with underacetylated isoforms of H3 and H4, suggesting that histone acetylation may modulate Tup1 functions in vivo. Here we report that histone hyperacetylation caused by combined mutations in genes encoding the histone deacetylases (HDACs) Rpd3, Hos1, and Hos2 abolishes Ssn6-Tup1 repression. Unlike HDAC mutations that do not affect repression, this combination of mutations causes concomitant hyperacetylation of both H3 and H4. Strikingly, two of these class I HDACs interact physically with Ssn6-Tup1. These findings suggest that Ssn6-Tup1 actively recruits deacetylase activities to deacetylate adjacent nucleosomes and promote Tup1-histone interactions.
摘要
Ssn6-Tup1调控酵母中的多个基因，为共抑制因子功能提供了一个范例。Tup1直接与组蛋白H3和H4相互作用，这些组蛋白的突变会协同损害Ssn6-Tup1介导的抑制作用。在体外，Tup1优先与H3和H4的低乙酰化异构体相互作用，这表明组蛋白乙酰化可能在体内调节Tup1的功能。在此我们报告，由编码组蛋白去乙酰化酶（HDAC）Rpd3、Hos1和Hos2的基因联合突变导致的组蛋白高度乙酰化消除了Ssn6-Tup1的抑制作用。与不影响抑制作用的HDAC突变不同，这种突变组合会导致H3和H4同时发生高度乙酰化。引人注目的是，这些I类HDAC中的两个与Ssn6-Tup1发生物理相互作用。这些发现表明，Ssn6-Tup1会主动募集去乙酰化酶活性，使相邻核小体去乙酰化，并促进Tup1与组蛋白的相互作用。
相似文献
1
Ssn6-Tup1 interacts with class I histone deacetylases required for repression.Ssn6-Tup1与抑制所需的I类组蛋白去乙酰化酶相互作用。
Genes Dev. 2000 Nov 1;14(21):2737-44. doi: 10.1101/gad.829100.
2
Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo.体内Tup1-Ssn6与受抑制基因的组蛋白依赖性关联。
Mol Cell Biol. 2002 Feb;22(3):693-703. doi: 10.1128/MCB.22.3.693-703.2002.
3
Tup1-Ssn6 interacts with multiple class I histone deacetylases in vivo.Tup1-Ssn6在体内与多种I类组蛋白去乙酰化酶相互作用。
J Biol Chem. 2003 Dec 12;278(50):50158-62. doi: 10.1074/jbc.M309753200. Epub 2003 Oct 2.
4
The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation.Set2甲基转移酶与Ssn6相关联，但Tup1 - Ssn6介导的基因抑制独立于组蛋白甲基化。
Biochem Biophys Res Commun. 2006 Jan 20;339(3):905-14. doi: 10.1016/j.bbrc.2005.11.103. Epub 2005 Nov 28.
5
Redundant mechanisms are used by Ssn6-Tup1 in repressing chromosomal gene transcription in Saccharomyces cerevisiae.在酿酒酵母中，Ssn6-Tup1利用多种冗余机制来抑制染色体基因转录。
J Biol Chem. 2004 Sep 17;279(38):39240-50. doi: 10.1074/jbc.M407159200. Epub 2004 Jul 14.
6
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.酵母全局阻遏物Tup1的阻遏结构域与组蛋白H3和H4直接相互作用。
Genes Dev. 1996 May 15;10(10):1247-59. doi: 10.1101/gad.10.10.1247.
7
Transcriptional repression by Tup1-Ssn6.由Tup1-Ssn6介导的转录抑制
Biochem Cell Biol. 2006 Aug;84(4):437-43. doi: 10.1139/o06-073.
8
Amino termini of histones H3 and H4 are required for a1-alpha2 repression in yeast.酵母中α1-α2抑制需要组蛋白H3和H4的氨基末端。
Mol Cell Biol. 1997 Nov;17(11):6555-62. doi: 10.1128/MCB.17.11.6555.
9
Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae.酿酒酵母中，Srb10细胞周期蛋白依赖性激酶和Hda1脱乙酰酶在Tup1介导的基因抑制中依赖启动子的作用。
Mol Biol Cell. 2004 Sep;15(9):4191-202. doi: 10.1091/mbc.e04-05-0412. Epub 2004 Jul 7.
10
Molecular genetic analysis of the yeast repressor Rfx1/Crt1 reveals a novel two-step regulatory mechanism.酵母阻遏物Rfx1/Crt1的分子遗传学分析揭示了一种全新的两步调控机制。
Mol Cell Biol. 2005 Sep;25(17):7399-411. doi: 10.1128/MCB.25.17.7399-7411.2005.
引用本文的文献
1
Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.功能表征与酵母多效核心抑制因子 Sin3、Cyc8 和 Tup1 相互作用的基因抑制介导结构域的比较分析。
Curr Genet. 2023 Jun;69(2-3):127-139. doi: 10.1007/s00294-023-01262-6. Epub 2023 Mar 1.
2
Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae.Tup1 在酿酒酵母静止期的转录抑制中至关重要。
PLoS Genet. 2022 Dec 21;18(12):e1010559. doi: 10.1371/journal.pgen.1010559. eCollection 2022 Dec.
3
Corepressors SsnF and RcoA Regulate Development and Aflatoxin B Biosynthesis in NRRL 3357.阻遏物 SsnF 和 RcoA 调控 NRRL 3357 中的生长发育和黄曲霉毒素 B 生物合成。
Toxins (Basel). 2022 Feb 25;14(3):174. doi: 10.3390/toxins14030174.
4
Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1-Cyc8 complex in Penicillium oxalicum and Trichoderma reesei.在草酸青霉和里氏木霉中，碳分解代谢物阻遏涉及转录因子CRE1/CreA与Tup1-Cyc8复合物的物理相互作用。
Biotechnol Biofuels. 2021 Dec 24;14(1):244. doi: 10.1186/s13068-021-02092-9.
5
Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.遗传分析表明，酿酒酵母 Tup1 核心抑制因子具有共激活子功能。
Genetics. 2021 Oct 2;219(2). doi: 10.1093/genetics/iyab120.
6
Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.转录抑制因子 Gal80 将核心抑制复合物 Cyc8-Tup1 招募到酿酒酵母 GAL 调控基因的结构基因上。
Curr Genet. 2022 Feb;68(1):115-124. doi: 10.1007/s00294-021-01215-x. Epub 2021 Oct 7.
7
Ash1 and Tup1 dependent repression of the Saccharomyces cerevisiae HO promoter requires activator-dependent nucleosome eviction.Ash1 和 Tup1 依赖性抑制酿酒酵母 HO 启动子需要激活剂依赖性核小体驱逐。
PLoS Genet. 2020 Dec 31;16(12):e1009133. doi: 10.1371/journal.pgen.1009133. eCollection 2020 Dec.
8
Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.通过着丝粒相关网络的组成型募集 Polo 激酶可提高着丝粒 RNA。
PLoS Genet. 2020 Aug 18;16(8):e1008990. doi: 10.1371/journal.pgen.1008990. eCollection 2020 Aug.
9
Regulated repression governs the cell fate promoter controlling yeast meiosis.调控抑制控制着控制酵母减数分裂的细胞命运启动子。
Nat Commun. 2020 May 8;11(1):2271. doi: 10.1038/s41467-020-16107-w.
10
Nut1/Hos1 and Sas2/Rpd3 control the H3 acetylation of two different sets of osmotic stress-induced genes.Nut1/Hos1 和 Sas2/Rpd3 控制两组不同的渗透压胁迫诱导基因的 H3 乙酰化。
Epigenetics. 2020 Mar;15(3):251-271. doi: 10.1080/15592294.2019.1664229. Epub 2019 Sep 12.
本文引用的文献
1
Steady-state levels of histone acetylation in Saccharomyces cerevisiae.酿酒酵母中组蛋白乙酰化的稳态水平。
J Biol Chem. 2000 Apr 28;275(17):13007-11. doi: 10.1074/jbc.275.17.13007.
2
Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme.Hrs1/Med3是RNA聚合酶II全酶中Cyc8-Tup1共抑制因子的作用靶点。
J Biol Chem. 2000 Mar 24;275(12):8397-403. doi: 10.1074/jbc.275.12.8397.
3
The organized chromatin domain of the repressed yeast a cell-specific gene STE6 contains two molecules of the corepressor Tup1p per nucleosome.被抑制的酵母a细胞特异性基因STE6的有序染色质结构域，每个核小体包含两个共抑制因子Tup1p分子。
EMBO J. 2000 Feb 1;19(3):400-9. doi: 10.1093/emboj/19.3.400.
4
5
In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment.用于在染色质环境中研究动态蛋白质：DNA相互作用的体内交联和免疫沉淀技术。
Methods. 1999 Nov;19(3):425-33. doi: 10.1006/meth.1999.0879.
6
Conservation of histone binding and transcriptional repressor functions in a Schizosaccharomyces pombe Tup1p homolog.粟酒裂殖酵母Tup1p同源物中组蛋白结合和转录抑制功能的保守性。
Mol Cell Biol. 1999 Dec;19(12):8461-8. doi: 10.1128/MCB.19.12.8461.
7
Yeast HOS3 forms a novel trichostatin A-insensitive homodimer with intrinsic histone deacetylase activity.酵母HOS3形成一种新型的对曲古抑菌素A不敏感的同型二聚体，具有内在的组蛋白脱乙酰酶活性。
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12356-61. doi: 10.1073/pnas.96.22.12356.
8
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.ATP 依赖的重塑和乙酰化作为染色质流动性的调节因子。
Genes Dev. 1999 Sep 15;13(18):2339-52. doi: 10.1101/gad.13.18.2339.
9
A functional interaction between the histone deacetylase Rpd3 and the corepressor groucho in Drosophila development.果蝇发育过程中组蛋白去乙酰化酶Rpd3与共抑制因子groucho之间的功能相互作用。
Genes Dev. 1999 Sep 1;13(17):2218-30. doi: 10.1101/gad.13.17.2218.
10
In vivo functions of histone acetylation/deacetylation in Tup1p repression and Gcn5p activation.组蛋白乙酰化/去乙酰化在Tup1p抑制和Gcn5p激活中的体内功能。
Cold Spring Harb Symp Quant Biol. 1998;63:459-68. doi: 10.1101/sqb.1998.63.459.
Zotero 插件