一种从稀释溶液中回收活性寡聚酶的简单方法。
A simple method for the recovery of active oligomeric enzyme from its diluted solutions.
作者信息
Joachimiak A, Barciszewski J
出版信息
J Biochem Biophys Methods. 1980 Apr;2(4):227-31. doi: 10.1016/0165-022x(80)90037-8.
PMID:7419869
Abstract
A method for the recovery of active enzyme from its diluted solutions is described. It includes batch sorption on DEAE-Sephadex A-50 which follows elution or chromatography of an enzyme on a DEAE-Sephasex A-50 column. All purified aminoacyl-tRNA synthetases retain high activity without degradation of the protein. The method is very fast and convenient.
摘要
本文描述了一种从稀释溶液中回收活性酶的方法。该方法包括在DEAE-葡聚糖A-50上进行分批吸附，此步骤在酶于DEAE-葡聚糖A-50柱上洗脱或层析之后。所有纯化的氨酰-tRNA合成酶均保持高活性，且蛋白质未发生降解。该方法非常快速且便捷。
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