Holbein B E, Jericho K W, Likes G C
Infect Immun. 1979 May;24(2):545-51. doi: 10.1128/iai.24.2.545-551.1979.
The influence of iron on Neisseria meningitidis infection in C-57 mice was examined. Iron sulfate, ferric ammonium citrate, and iron sorbitol citrate all proved to be too toxic for use as infection-enhancing agents. Iron dextran displayed an extremely low toxicity, enhanced infection in a dose-dependent manner, and resulted in infection enhancement factors approaching 10(9) for virulent strains of N. meningitidis. Fatal iron dextran-enhanced infection was shown to be reversible by in vivo chelation of iron. Virulent strains of N. meningitidis produced symptoms of infection and pathological lesions in mice both with and without iron pretreatment, but an avirulent strain failed to produce symptoms of infection or pathological lesions, regardless of iron administration. Iron dextran-enhanced infection in mice proved to be a useful model for the examination of virulence of various N. meningitidis strains. All of 9 isolates from clinical disease possessed virulence, whereas only 3 of 10 isolates from carriers possessed virulence, when examined by using this model.
研究了铁对C-57小鼠脑膜炎奈瑟菌感染的影响。硫酸铁、枸橼酸铁铵和枸橼酸铁山梨醇用作感染增强剂时毒性均过大。右旋糖酐铁毒性极低，以剂量依赖方式增强感染，对于脑膜炎奈瑟菌的强毒株，感染增强因子接近10(9)。体内螯合铁可使右旋糖酐铁增强的致命感染逆转。脑膜炎奈瑟菌的强毒株在有或没有铁预处理的小鼠中均产生感染症状和病理损伤，但无毒株无论是否给予铁均未产生感染症状或病理损伤。右旋糖酐铁增强小鼠感染被证明是检测各种脑膜炎奈瑟菌菌株毒力的有用模型。当用该模型检测时，临床疾病的9株分离株均具有毒力，而携带者的10株分离株中只有3株具有毒力。
