Jann B, Reske K, Jann K
Eur J Biochem. 1975 Dec 1;60(1):239-46. doi: 10.1111/j.1432-1033.1975.tb20996.x.
Lipopolysaccharide preparations from R(rough) Escherichia coli O8-,SR(semirough) Salmonella typhimurium and S (smooth) strains E. coli O8 and Citrobacter 396 were disintegrated with sodium dodecylsulfate and subjected to polyacrylamide gel electrophoresis in the presence of 1% sodium dodecylsulfate. The results obtained were compared with those obtained from the same lipopolysaccharide preparations by degradation analysis. In dodecylsulfate gel electrophoresis the lipopolysaccharide preparation from the E. coli R mutant and the S. typhimurium SR mutant showed one band each (R-and SR-band, respectively) with different electrophoretic mobilities. The lipopolysaccharide preparations from the E. coli O8-strain exhibited two bands, one of which had the same electrophoretic mobility as the R-band and the other was identified as S-band. The lipopolysaccharide preparation from the Citrobacter 396-S-strain exhibited four bands: one R-band, one SR-band and two S-bands. The results showed that wild-type S strains contain more than one type of lipopolysaccharide. They differ in the length of their O-specific polysaccharide chains. The lipid A content of the different lipopolysaccharide was expressed in their electrophoretic mobilities.
用十二烷基硫酸钠将来自粗糙型大肠杆菌O8 - 、半粗糙型鼠伤寒沙门氏菌以及光滑型大肠杆菌O8和柠檬酸杆菌396的脂多糖制剂进行裂解，并在1%十二烷基硫酸钠存在的条件下进行聚丙烯酰胺凝胶电泳。将所得结果与通过降解分析从相同脂多糖制剂中获得的结果进行比较。在十二烷基硫酸钠凝胶电泳中，大肠杆菌R突变体和鼠伤寒沙门氏菌SR突变体的脂多糖制剂分别显示出一条带（分别为R带和SR带），其电泳迁移率不同。大肠杆菌O8菌株的脂多糖制剂显示出两条带，其中一条带的电泳迁移率与R带相同，另一条被鉴定为S带。柠檬酸杆菌396 - S菌株的脂多糖制剂显示出四条带：一条R带、一条SR带和两条S带。结果表明，野生型S菌株含有不止一种类型的脂多糖。它们的O - 特异性多糖链长度不同。不同脂多糖的脂质A含量在其电泳迁移率中得以体现。
