Hitchcock P J
Eur J Biochem. 1983 Jul 1;133(3):685-8. doi: 10.1111/j.1432-1033.1983.tb07517.x.
Purified lipopolysaccharides of salmonellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre-electrophoresis of polyacrylamide gels had no apparent effect on one-dimensional silver-stained lipopolysaccharide profiles. However, without pre-electrophoresis, two-dimensional and three-dimensional patterns contained numerous bands with varied migration patterns compared to those in the one-dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre-electrophoresis of gels eliminated aberrant migration patterns.
通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分离沙门氏菌菌株的纯化脂多糖。聚丙烯酰胺凝胶的预电泳对一维银染脂多糖图谱没有明显影响。然而，在没有预电泳的情况下，与一维凝胶相比，二维和三维图谱包含许多具有不同迁移模式的条带。脂多糖在电泳过程中在聚丙烯酰胺凝胶内发生了改变。凝胶的预电泳消除了异常的迁移模式。
