Castellini C, Lattaioli P, Bernardini M, Dal Bosco A
Department of Animal Science, Borgo 20 Giugno, Perugia, Italy.
Theriogenology. 2000 Sep 1;54(4):523-33. doi: 10.1016/S0093-691X(00)00367-8.
The objective of this research was to verify the effects of dietary alpha-tocopheryl acetate (50 vs. 200 mg/kg diet) and ascorbic acid (0 vs. 1 g/L water) on the relative amounts on semen and motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa stored for 24 h at 5 degrees C. A high amount of dietary (alpha-tocopheryl acetate significantly increased the level of Vitamin E in the semen (0.90 vs. 0.41 micromol/L) and its oxidative stability after storage (Thiobarbituric Acid Reactive Substances-- TBARS 15.88 vs. 20.90 nmol Malondialdehyde--MDA/mL). Ascorbic acid showed a different effect in relation to the Vitamin E status of animals: when associated with the higher level of Vitamin E it increased the (alpha-tocopherol and the oxidative stability of semen (2.67 micromol/L and 12.25 nmol MDA/mL, respectively), whereas both parameters were reduced with lower Vitamin E (0.13 micromol/L and 21.20 nmol MDA/mL). Semen traits were not modified by the separate supplementation of supranutritional levels of vitamins, whereas their combination significantly improved the viability and the kinetics of spermatozoa (e.g. track speed: 95.13 vs. 71.31 microm sec(-1)) with an increase in fertility rate (70.0 vs. 63.3; P=0.06) that could be considered almost significant.
本研究的目的是验证日粮中醋酸生育酚（50 与 200 毫克/千克日粮）和抗坏血酸（0 与 1 克/升水）对 5℃下储存 24 小时的兔精子的精液相对含量、运动特性、氧化稳定性和受精能力的影响。日粮中高剂量的醋酸生育酚显著提高了精液中维生素 E 的水平（0.90 与 0.41 微摩尔/升）及其储存后的氧化稳定性（硫代巴比妥酸反应性物质——TBARS，15.88 与 20.90 纳摩尔丙二醛——MDA/毫升）。抗坏血酸对动物的维生素 E 状态显示出不同的影响：当与较高水平的维生素 E 联合使用时，它提高了α-生育酚和精液的氧化稳定性（分别为 2.67 微摩尔/升和 12.25 纳摩尔 MDA/毫升），而当维生素 E 水平较低时，这两个参数均降低（0.13 微摩尔/升和 21.20 纳摩尔 MDA/毫升）。超营养水平维生素的单独添加未改变精液特性，而它们的组合显著提高了精子的活力和运动学参数（例如轨迹速度：95.13 与 71.31 微米/秒），且受精率有所提高（70.0 与 63.3；P = 0.06），可认为几乎具有显著性。
