Castellini Cesare, Lattaioli Paolo, Bosco Alessandro Dal, Beghelli Daniela
Department of Animal Science, Borgo 20 Giugno, 74-06100 Perugia, Italy.
Theriogenology. 2002 Dec;58(9):1723-32. doi: 10.1016/s0093-691x(02)01083-x.
This research examined the effects of dietary alpha-tocopheryl acetate (50 or 200 mg/kg diet) and selenium (Se, 0 or 0.5 ppm) supplementation on motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa, fresh and stored for 24 h at 5 degrees C. The higher amount of dietary alpha-tocopheryl acetate increased the level of Vitamin E in the fresh semen (1.75 mmol/l versus 0.95 mmol/l) and its oxidative stability (thiobarbituric acid reactive substances-TBARS 12.44 nmol malondialdehyde/10(8) sperm versus 21.4 nmol malondialdehyde/10(8) sperm). Dietary Se increased gluthatione peroxidase activity (GPx) in erythrocytes (285 U/g Hb versus 207 U/g Hb), seminal plasma (270 U/l versus 190 U/l) and spermatozoa (1338 mU/10(9) sperm versus 1103 mU/10(9) sperm), whereas it did not show any effect on alpha-tocopherol level and TBARS. No synergy between Vitamin E and Se was shown. Storage for 24 h at 5 degrees C increased the TBARS level in all the experimental groups. Neither live and acrosome reacted spermatozoa, nor kinetic parameters, nor fertility rate were modified by dietary supplementation.
本研究检测了日粮中添加醋酸生育酚（50或200毫克/千克日粮）和硒（Se，0或0.5 ppm）对家兔精子运动特性、氧化稳定性和受精能力的影响，这些精子分别为新鲜的以及在5℃下保存24小时后的。日粮中较高剂量的醋酸生育酚提高了新鲜精液中维生素E的水平（1.75毫摩尔/升对0.95毫摩尔/升）及其氧化稳定性（硫代巴比妥酸反应物质-TBARS，12.44纳摩尔丙二醛/10⁸精子对21.4纳摩尔丙二醛/10⁸精子）。日粮中的硒提高了红细胞（285 U/g血红蛋白对207 U/g血红蛋白）、精浆（270 U/升对190 U/升）和精子（1338毫微单位/10⁹精子对1103毫微单位/10⁹精子）中的谷胱甘肽过氧化物酶活性（GPx），而对维生素E水平和TBARS没有任何影响。未显示维生素E和硒之间有协同作用。在5℃下保存24小时会使所有实验组的TBARS水平升高。日粮添加对活的和顶体反应的精子、动力学参数或受精率均无影响。
