一氧化氮在促肾上腺皮质激素诱导的高血压中的作用：L-精氨酸的作用被N-硝基-L-精氨酸逆转。

Role of nitric oxide in adrenocorticotrophin-induced hypertension: L-arginine effects reversed by N-nitro-L-arginine.

Wen C, Li M, Whitworth J A

Department of Medicine, St George Hospital, The University of New South Wales, Sydney, Australia.

Clin Exp Pharmacol Physiol. 2000 Nov;27(11):887-90. doi: 10.1046/j.1440-1681.2000.03354.x.

Abstract

L-arginine prevents adrenocorticotrophin (ACTH)-induced hypertension in the rat. To confirm that this effect is mediated through the nitric oxide (NO) system, we examined whether N-nitro-L-arginine (NOLA) could reverse the L-arginine-induced blockade of ACTH-induced hypertension. 2. Blood pressure and metabolic parameters were examined in sham-, ACTH-, L-arginine + sham-, NOLA + sham-, ACTH + L-arginine- and ACTH + L-arginine + NOLA-treated Sprague-Dawley rats (n = 40). 3. Adrenocorticotrophin treatment increased systolic blood pressure (SBP), water intake and urine output and decreased bodyweight. N-Nitro-L-arginine alone increased SBP without affecting metabolic variables. L-Arginine alone did not affect blood pressure. The SBP was lower in L-arginine + ACTH- than ACTH-treated rats (P < 0.001), but was higher following ACTH + L-arginine + NOLA than ACTH + L-arginine (P < 0.05). 4. N-Nitro-L-arginine reversed the blood pressure-lowering effect of L-arginine in ACTH-induced hypertension in the rat, supporting the notion that NO plays a role in the hypertension.

摘要

L-精氨酸可预防大鼠促肾上腺皮质激素（ACTH）诱导的高血压。为证实这种作用是通过一氧化氮（NO）系统介导的，我们研究了N-硝基-L-精氨酸（NOLA）是否能逆转L-精氨酸对ACTH诱导的高血压的阻断作用。2. 对假手术组、ACTH组、L-精氨酸+假手术组、NOLA+假手术组、ACTH+L-精氨酸组和ACTH+L-精氨酸+NOLA组处理的Sprague-Dawley大鼠（n = 40）进行血压和代谢参数检测。3. ACTH处理可使收缩压（SBP）升高、水摄入量和尿量增加，并使体重下降。单独使用N-硝基-L-精氨酸可使SBP升高，但不影响代谢变量。单独使用L-精氨酸对血压无影响。L-精氨酸+ACTH组大鼠的SBP低于ACTH处理组大鼠（P < 0.001），但ACTH+L-精氨酸+NOLA组大鼠的SBP高于ACTH+L-精氨酸组（P < 0.05）。4. N-硝基-L-精氨酸逆转了L-精氨酸对大鼠ACTH诱导的高血压的降压作用，支持了NO在高血压中起作用的观点。