大肠杆菌碱性磷酸酶调控突变体的遗传分析。
Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli.
作者信息
Kreuzer K, Pratt C, Torriani A
出版信息
Genetics. 1975 Nov;81(3):459-68. doi: 10.1093/genetics/81.3.459.
Abstract
A fine structure map of the phoR region of E. coli, mutations of which affect the rate of alkaline phosphatase synthesis, was constructed by Hfr X F- crosses. Mutations causing three different phenotypes (previously reported as phoRa, phoRb, phoRc (Garen and Echols 1962a,b) are clustered in three closely linked genetic loci. PhoR mutants of all three types, including the phoRb type not previously tested, are recessive to wild-type phoR+. In addition, phoRa and phoRc complement each other, while phoRa and phoRb do not. Our results support the hypothesis of Morris et al. (1974) that phoRc mutants represent a cistron (phoB) different from phoR.
摘要
通过高频重组（Hfr）菌株与F-菌株杂交构建了大肠杆菌phoR区域的精细结构图谱，该区域的突变会影响碱性磷酸酶的合成速率。导致三种不同表型（先前报道为phoRa、phoRb、phoRc，Garen和Echols，1962a，b）的突变聚集在三个紧密连锁的基因位点。所有三种类型的PhoR突变体，包括之前未测试过的phoRb类型，相对于野生型phoR+都是隐性的。此外，phoRa和phoRc相互互补，而phoRa和phoRb则不互补。我们的结果支持了Morris等人（1974）的假设，即phoRc突变体代表一个与phoR不同的顺反子（phoB）。
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