Kitajima T, Oishi E, Amimoto K, Satoshi U, Nakamura H, Oda K, Katayama S, Izumida A, Shimizu Y
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Uji, Japan.
J Vet Med Sci. 2000 Oct;62(10):1073-7. doi: 10.1292/jvms.62.1073.
Mouse monoclonal antibodies (MAbs), raised against the NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain Kyoto (serovar 2), recognized two different epitopes on a single protein of molecular weight 67 kDa. The MAbs were classified as protective or non-protective against strain Fujisawa (serovar 1). In immunoblotting analysis using the MAbs, fifteen wild strains were shown to contain different amounts of 67 kDa protective antigen. Each formalin-killed whole cell vaccine (bacterin) prepared from the fifteen wild strains conferred different levels of protection against strain Fujisawa in mice. Bacterins prepared from wild strains with larger amounts of 67 kDa protective antigen tended to give high levels of antigen-specific antibody and better protection to mice. These results indicate that the amount of 67 kDa protective antigen which influences the induction of protective immune responses may vary substantially among the strains of E. rhusiopathiae (serovar 2).
针对豕丹毒京都菌株（血清型2）经氢氧化钠提取的抗原产生的小鼠单克隆抗体（MAbs），识别出一种分子量为67 kDa的单一蛋白质上的两种不同表位。这些单克隆抗体被分类为对藤泽菌株（血清型1）具有保护性或非保护性。在使用这些单克隆抗体的免疫印迹分析中，15株野生菌株显示含有不同量的67 kDa保护性抗原。从这15株野生菌株制备的每种福尔马林灭活全细胞疫苗（菌苗）在小鼠中对藤泽菌株提供了不同水平的保护。由含有较多67 kDa保护性抗原的野生菌株制备的菌苗往往能产生高水平的抗原特异性抗体，并对小鼠提供更好的保护。这些结果表明，影响保护性免疫反应诱导的67 kDa保护性抗原的量在豕丹毒（血清型2）菌株之间可能有很大差异。
