Kobayashi S, Sato H, Hirose K, Saito H
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Vet Microbiol. 1992 Jan;30(1):73-85. doi: 10.1016/0378-1135(92)90095-b.
Culture filtrate and alkaline-extracted antigens from whole cells of an attenuated strain of Erysipelothrix rhusiopathiae (strain Koganei: serovar 1a) were fractionated with ammonium sulfate; both induced protective immunity in mice. Sephadex G-200 gel filtration revealed three protein fractions in the alkaline-extracted antigen and four protein fractions in the culture filtrate antigen. A fraction in the alkaline extract (NaOH P-2) and in the culture filtrate (CF P-2) induced protection in mice against challenge with a different serovar strain (strain Agata: serovar 5). Anti-NaOH P-2 and anti-CF P-2 mouse sera were protective against different serovars. Glycoprotein fraction derived from CF P-2 antigen by affinity chromatography with Con A-Sepharose 4B did not show protective activity. Western blotting between the antisera (anti-NaOH P-2, Anti-CF P-2 and anti-Koganei strain) and the antigens (NaOH P-2, and sonicated antigens of Agata, Fujisawa and Koganei strains) showed strong recognition of the same bands at 62, 42 and 41 kDa.
对猪丹毒杆菌减毒株（小金井株：血清型1a）的全细胞培养滤液和碱提取抗原进行硫酸铵分级分离；二者均可诱导小鼠产生保护性免疫。Sephadex G - 200凝胶过滤显示碱提取抗原中有三个蛋白组分，培养滤液抗原中有四个蛋白组分。碱提取物中的一个组分（NaOH P - 2）和培养滤液中的一个组分（CF P - 2）可诱导小鼠对不同血清型菌株（阿加塔株：血清型5）的攻击产生保护作用。抗NaOH P - 2和抗CF P - 2小鼠血清对不同血清型具有保护作用。用Con A - Sepharose 4B亲和层析从CF P - 2抗原中获得的糖蛋白组分未显示出保护活性。抗血清（抗NaOH P - 2、抗CF P - 2和抗小金井株血清）与抗原（NaOH P - 2以及阿加塔株、藤泽株和小金井株的超声破碎抗原）之间的蛋白质印迹分析显示，在62、42和41 kDa处有对相同条带的强烈识别。
