由猪丹毒丝菌全细胞经碱性处理及从其培养滤液制备的保护性抗原的免疫学特性

Immunological characterization of protective antigens prepared by alkaline treatment of whole cells and from the culture filtrate of Erysipelothrix rhusiopathiae.

作者信息

Kobayashi S, Sato H, Hirose K, Saito H

机构信息

Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.

出版信息

Vet Microbiol. 1992 Jan;30(1):73-85. doi: 10.1016/0378-1135(92)90095-b.

DOI:10.1016/0378-1135(92)90095-b

PMID:1531564

Abstract

Culture filtrate and alkaline-extracted antigens from whole cells of an attenuated strain of Erysipelothrix rhusiopathiae (strain Koganei: serovar 1a) were fractionated with ammonium sulfate; both induced protective immunity in mice. Sephadex G-200 gel filtration revealed three protein fractions in the alkaline-extracted antigen and four protein fractions in the culture filtrate antigen. A fraction in the alkaline extract (NaOH P-2) and in the culture filtrate (CF P-2) induced protection in mice against challenge with a different serovar strain (strain Agata: serovar 5). Anti-NaOH P-2 and anti-CF P-2 mouse sera were protective against different serovars. Glycoprotein fraction derived from CF P-2 antigen by affinity chromatography with Con A-Sepharose 4B did not show protective activity. Western blotting between the antisera (anti-NaOH P-2, Anti-CF P-2 and anti-Koganei strain) and the antigens (NaOH P-2, and sonicated antigens of Agata, Fujisawa and Koganei strains) showed strong recognition of the same bands at 62, 42 and 41 kDa.

摘要

对猪丹毒杆菌减毒株（小金井株：血清型1a）的全细胞培养滤液和碱提取抗原进行硫酸铵分级分离；二者均可诱导小鼠产生保护性免疫。Sephadex G - 200凝胶过滤显示碱提取抗原中有三个蛋白组分，培养滤液抗原中有四个蛋白组分。碱提取物中的一个组分（NaOH P - 2）和培养滤液中的一个组分（CF P - 2）可诱导小鼠对不同血清型菌株（阿加塔株：血清型5）的攻击产生保护作用。抗NaOH P - 2和抗CF P - 2小鼠血清对不同血清型具有保护作用。用Con A - Sepharose 4B亲和层析从CF P - 2抗原中获得的糖蛋白组分未显示出保护活性。抗血清（抗NaOH P - 2、抗CF P - 2和抗小金井株血清）与抗原（NaOH P - 2以及阿加塔株、藤泽株和小金井株的超声破碎抗原）之间的蛋白质印迹分析显示，在62、42和41 kDa处有对相同条带的强烈识别。