Groschup M H, Cussler K, Weiss R, Timoney J F
Department of Microbiology, Immunology and Parasitology, NYSCVM, Cornell University, Ithaca 14850.
Epidemiol Infect. 1991 Dec;107(3):637-49. doi: 10.1017/s0950268800049335.
Although vaccination is widely practiced against infection by Erysipelothrix rhusiopathiae in pigs and turkeys, the protective antigen(s) involved have not been fully characterized or purified to homogeneity. Antigens of E. rhusiopathiae strain T28, serotype 2b, and of FRANKFURT XI, serotype N, in culture supernatant and in extracts made with hot acid, 10 mM NaOH, ultrasound or EDTA were compared by SDS-PAGE and immunoblotting and in a mouse protection test. EDTA and 10 mM NaOH yielded highly protective extracts; culture supernatant was less protective and ultrasonic or hot acid extracts stimulated little or no protection in mice. Protective antisera from swine, horses and mice recognized prominent bands of molecular mass (m.m.) of 66-64 and 40-39 kDa in EDTA and 10 mM NaOH extracts. Mice immunized with preparations of the 66-64 kDa band purified by preparative electrophoresis were protected. Both antigens were trypsin sensitive, contained no detectable polysaccharide, and showed a marked tendency to aggregate in the absence of SDS.
虽然在猪和火鸡中广泛进行针对猪丹毒丝菌感染的疫苗接种，但所涉及的保护性抗原尚未得到充分表征或纯化至同质。通过SDS-PAGE、免疫印迹以及小鼠保护试验，对猪丹毒丝菌T28菌株（血清型2b）和FRANKFURT XI菌株（血清型N）在培养上清液以及用热酸、10 mM NaOH、超声或EDTA制备的提取物中的抗原进行了比较。EDTA和10 mM NaOH产生了高度保护性的提取物；培养上清液的保护性较差，超声或热酸提取物在小鼠中几乎没有或没有刺激产生保护作用。来自猪、马和小鼠的保护性抗血清在EDTA和10 mM NaOH提取物中识别出分子量为66 - 64 kDa和40 - 39 kDa的显著条带。用通过制备性电泳纯化的66 - 64 kDa条带的制剂免疫的小鼠受到了保护。两种抗原对胰蛋白酶敏感，不含可检测到的多糖，并且在没有SDS的情况下显示出明显的聚集倾向。
