Rodriguez R L, Davern C I
J Bacteriol. 1976 Jan;125(1):346-52. doi: 10.1128/jb.125.1.346-352.1976.
The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chloramphenicol. Therefore, although the rate of replication can be affected by certain physiological stimuli, the topology of replication need not be.
通过放射自显影法显示，大肠杆菌温度敏感起始突变体（CT28）中的染色体复制方向是双向的。即使在琥珀酸基本培养基中缓慢生长或存在氯霉素时复制速率降低，这种复制模式仍然持续。因此，虽然复制速率会受到某些生理刺激的影响，但复制的拓扑结构不必受其影响。
