在大肠杆菌B-r同步培养物中氯霉素存在情况下的残留胸苷掺入。
Residual thymidine incorporation in the presence of chloramphenicol in synchronous cultures of Escherichia coli B-r.
作者信息
Helmstetter C E
出版信息
J Bacteriol. 1974 Oct;120(1):565-7. doi: 10.1128/jb.120.1.565-567.1974.
Abstract
Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an estimate of the length of the D period rather than the time of initiation of chromosome replication.
摘要
将细胞分裂间隔时间分别为45分钟和85分钟的同步生长的大肠杆菌B/r ATCC 12407，每隔一段时间暴露于氯霉素（200微克/毫升）和[¹⁴C]胸腺嘧啶核苷中，暴露时间有短有长（剩余时间）。结果表明，85分钟细胞中剩余胸腺嘧啶核苷掺入模式得出的是D期长度的估计值，而非染色体复制起始时间的估计值。
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本文引用的文献
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Analysis of the chloramphenicol-sensitive and chloramphenicol-resistant steps in the initiation of DNA synthesis in E. coli B-r.大肠杆菌B-r中DNA合成起始过程中氯霉素敏感和耐药步骤的分析。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):905-12. doi: 10.1073/pnas.64.3.905.
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The regulation of DNA replication and cell division in E. coli B-r.大肠杆菌B-r中DNA复制和细胞分裂的调控
Cold Spring Harb Symp Quant Biol. 1968;33:823-38. doi: 10.1101/sqb.1968.033.01.094.
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DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r.快速生长的大肠杆菌B/r分裂周期中的DNA合成
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Initiation of chromosome replication in Escherichia coli. I. Requirements for RNA and protein synthesis at different growth rates.大肠杆菌中染色体复制的起始。I. 不同生长速率下对RNA和蛋白质合成的需求。
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