Sugauchi F, Mizokami M, Orito E, Ohno T, Hayashi K, Kato T, Tanaka Y, Kato H, Ueda R
Second Department of Internal Medicine/Blood Transfusion, Nagoya City University Medical School, Nagoya, Japan.
Clin Infect Dis. 2000 Nov;31(5):1195-201. doi: 10.1086/317428. Epub 2000 Nov 7.
To elucidate needlestick transmission of hepatitis B virus (HBV), strains isolated from 1 physician who acquired HBV infection through a needlestick accident and 3 patients with chronic hepatitis B (donor patients A, B, and C) were tested using molecular evolutionary analysis based on full-length HBV genomic sequences. Nucleotide sequences of these isolates were aligned with 55 previously reported full-length genomic sequences. Genetic distances were estimated using the 6-parameter method, and phylogenetic trees were constructed using the neighbor-joining method. Strains isolated from patient A and the recipient pair were clustered within a closer range of evolutionary distances than were strains recovered from the recipient pair and patients B and C. Furthermore, strains from patient A and the recipient were also clustered on the S gene sequences of HBV. These results demonstrated that patient A alone was the source of direct transmission to the recipient. This approach can be used to investigate the transmission route of HBV.
为阐明乙型肝炎病毒（HBV）的针刺传播情况，我们对1名因针刺事故感染HBV的医生以及3例慢性乙型肝炎患者（供体患者A、B和C）分离出的毒株进行了检测，检测采用基于全长HBV基因组序列的分子进化分析方法。将这些分离株的核苷酸序列与之前报道的55个全长基因组序列进行比对。使用六参数法估计遗传距离，并采用邻接法构建系统发育树。与从受体对以及患者B和C分离出的毒株相比，从患者A和受体对分离出的毒株在更近的进化距离范围内聚类。此外，来自患者A和受体的毒株在HBV的S基因序列上也聚类。这些结果表明，仅患者A是直接传播给受体的源头。这种方法可用于研究HBV的传播途径。
