Van Haeften T, Wouterlood F G
Department of Anatomy, Faculty of Medicine, Graduate School Neurosciences Amsterdam, Institute for Neurosciences Vrije Universiteit, Amsterdam, The Netherlands.
J Neurosci Methods. 2000 Nov 15;103(1):107-16. doi: 10.1016/s0165-0270(00)00300-9.
Most techniques used for the study of the fiber connectivity in the central nervous system produce results which are visualized in the conventional light microscope or fluorescence microscope. Although in some cases this may be sufficient, often proof is necessary that fibers which enter a particular brain area indeed terminate here. Alternatively, it may be necessary to determine whether the axon terminals of traced fibers form synapses with specific processes of specific neurons. With the latter neurons all cellular elements are meant which can be labeled in some way. Evidence of synaptic connectivity necessitates visualization at a higher level of resolution, that is at the electron-microscopic level. In this contribution to the Special Issue we discuss several methods currently available to visualize individual tracers, and methods developed to visualize two different markers, that is one marker attached to a fiber or an axon terminal, and the second marker attached to a presumed pre- or postsynaptic neuronal element.
大多数用于研究中枢神经系统纤维连接性的技术所产生的结果，是在传统光学显微镜或荧光显微镜下可视化呈现的。尽管在某些情况下这可能足够了，但通常有必要证明进入特定脑区的纤维确实在此处终止。或者，可能有必要确定追踪纤维的轴突终末是否与特定神经元的特定突起形成突触。对于后一种神经元而言，所有可以以某种方式标记的细胞成分都涵盖在内。突触连接的证据需要在更高分辨率水平上进行可视化，即电子显微镜水平。在本特刊的这篇稿件中，我们讨论了目前可用于可视化单个示踪剂的几种方法，以及为可视化两种不同标记物而开发的方法，即一种标记物附着于纤维或轴突终末，另一种标记物附着于假定的突触前或突触后神经元成分。
