Heinsen H, Arzberger T, Schmitz C
Morphological Brain Research Unit, University of Wuerzburg, Josef-Schneider-Strasse 2, D-97080, Wuerzburg, Germany.
J Chem Neuroanat. 2000 Oct;20(1):49-59. doi: 10.1016/s0891-0618(00)00067-3.
Celloidin mounting (embedding without infiltration) of the human central nervous system (CNS) proved to be superior to gelatin embedding for the production of serial sections ranging in thickness from 220 to 500 microm. After gallocyanin-staining, a comprehensive neuroanatomical as well as neuropathological survey of the human brain is possible, including diagnosis of Alzheimer's disease. Details of a fractionator analysis of the total striatal neuron number are described and the possible quantitative analysis of parallel immunohistochemically stained sections is discussed.
对于制作厚度在220至500微米之间的连续切片而言，人中枢神经系统（CNS）的火棉胶包埋（不经过渗透的包埋）被证明优于明胶包埋。经间苯三酚染色后，对人脑进行全面的神经解剖学以及神经病理学检查成为可能，包括阿尔茨海默病的诊断。文中描述了对纹状体神经元总数进行分馏分析的细节，并讨论了对平行免疫组织化学染色切片进行定量分析的可能性。
