Hogers B, Gross D, Lehmann V, Zick K, De Groot H J, Gittenberger-De Groot A C, Poelmann R E
Department of Anatomy, Leiden University Medical Center, Leiden, The Netherlands.
Anat Rec. 2000 Dec 1;260(4):373-7. doi: 10.1002/1097-0185(20001201)260:4<373::AID-AR60>3.0.CO;2-Q.
Magnetic resonance microscopy (MRM) was used to study mouse embryonic development in utero. MRM is a non-invasive imaging technique to study normal and abnormal embryonic development. To overcome image blurring as a result of embryonic movement, fast imaging sequences were used (less than 1 min scanning time). Clear morphologic proton images were obtained by diffusion spin echo and by rapid acquisition with relaxation enhancement (RARE), revealing living mouse embryos with great anatomical detail. In addition, functional information about embryonic blood flow could be obtained, in the absence of a contrast agent. This was achieved by combining two imaging sequences, RARE and very fast gradient echo. We expect that MRM will soon become a feasible method to study longitudinally both normal and abnormal (transgenic) mouse development.
磁共振显微镜（MRM）被用于研究子宫内的小鼠胚胎发育。MRM是一种用于研究正常和异常胚胎发育的非侵入性成像技术。为了克服胚胎运动导致的图像模糊，使用了快速成像序列（扫描时间少于1分钟）。通过扩散自旋回波和弛豫增强快速采集（RARE）获得了清晰的形态学质子图像，揭示了具有详细解剖结构的活体小鼠胚胎。此外，在不使用造影剂的情况下，可以获得有关胚胎血流的功能信息。这是通过结合两种成像序列，即RARE和极快速梯度回波实现的。我们预计，MRM很快将成为一种可行的方法，用于纵向研究正常和异常（转基因）小鼠的发育。
