Smith B R, Linney E, Huff D S, Johnson G A
Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA.
Comput Med Imaging Graph. 1996 Nov-Dec;20(6):483-90. doi: 10.1016/s0895-6111(96)00046-8.
We demonstrate that magnetic resonance (MR) microscopy provides a mechanism to investigate normal and abnormal developmental anatomy in a non-destructive and distortion-free manner. Techniques for the fixation, embedding, perfusion and image acquisition of embryos between 3 and 30 mm crown rump length are described. We describe the perfusion of a contrast agent to enhance images of the developing embryonic vasculature. Data are acquired as three-dimensional isotropic arrays which permit images to be reformatted retrospectively in any plane. The data are available for archiving, distributing and for post-acquisition manipulations. MR microscopy is a fast technique for producing three-dimensional reconstructions and is free from registration and sectioning artifacts.
我们证明，磁共振（MR）显微镜提供了一种以无损且无失真的方式研究正常和异常发育解剖结构的机制。本文描述了针对头臀长在3至30毫米之间的胚胎进行固定、包埋、灌注及图像采集的技术。我们描述了灌注造影剂以增强发育中胚胎脉管系统图像的方法。数据以三维各向同性阵列形式采集，这使得图像能够在任何平面上进行回顾性重新格式化。这些数据可用于存档、分发以及采集后处理。MR显微镜是一种用于生成三维重建的快速技术，且不存在配准和切片伪影。
