Leao J C, Kumar N, McLean K A, Porter S R, Scully C M, Swan A V, Teo C G
Departamento de Clínica e Odontologia Preventiva, Universidade Federal de Pernambuco, Recife, Brazil.
J Med Virol. 2000 Dec;62(4):416-20. doi: 10.1002/1096-9071(200012)62:4<416::aid-jmv4>3.0.co;2-z.
The effect of human immunodeficiency virus-1 protease inhibitors on the frequency of human herpesvirus 8 DNA detection from peripheral blood of human immunodeficiency virus-positive persons was evaluated. Thirty-three human immunodeficiency virus-seropositive male patients were studied longitudinally. DNA from open reading frame 26 of the human herpesvirus 8 genome was amplified by the polymerase chain reaction from the CD45+ fraction of peripheral blood before and after the introduction of protease inhibitor therapy. Human herpesvirus 8 IgG status, CD4+ cell counts, and human immunodeficiency virus-1 plasma viral load were also assessed before and after therapy. When both reverse transcriptase inhibitor and protease inhibitor treatment were introduced at the same time, there was an increase in CD4+ T cell counts (P=0.0041), a decrease in human immunodeficiency virus plasma load (P=0.0584), and a decrease in the detection rate of human herpesvirus 8 DNA (P=0.0077). Introducing protease inhibitor to patients already receiving reverse transcriptase inhibitor treatment was associated with an increase in CD4+ T cell counts (P=0.0003), a decrease in human immunodeficiency virus plasma viral load (P=0.0911), and a decrease in the human herpesvirus 8 detection rate (P=0.0412). No significant changes in the titters of anti-human herpesvirus 8 IgG were observed. Treatment with human immunodeficiency virus-1 protease inhibitors is therefore associated with the clearance of human herpesvirus 8 DNA from peripheral blood of human immunodeficiency virus-infected patients. The concomitant decrease in the human immunodeficiency virus plasma load and increase in the peripheral CD4+ cell count suggest that an amelioration in the immune defect following reduction in the burden of human immunodeficiency virus-1 infection is responsible for the clearance of human herpesvirus 8 by protease inhibitors.
评估了人类免疫缺陷病毒1型蛋白酶抑制剂对人类免疫缺陷病毒阳性者外周血中人类疱疹病毒8型DNA检测频率的影响。对33名人类免疫缺陷病毒血清阳性男性患者进行了纵向研究。在引入蛋白酶抑制剂治疗前后，通过聚合酶链反应从外周血的CD45 +部分扩增人类疱疹病毒8型基因组开放阅读框26的DNA。还在治疗前后评估了人类疱疹病毒8型IgG状态、CD4 +细胞计数和人类免疫缺陷病毒1型血浆病毒载量。当同时引入逆转录酶抑制剂和蛋白酶抑制剂治疗时，CD4 + T细胞计数增加（P = 0.0041），人类免疫缺陷病毒血浆载量降低（P = 0.0584），人类疱疹病毒8型DNA检测率降低（P = 0.0077）。对已经接受逆转录酶抑制剂治疗的患者引入蛋白酶抑制剂与CD4 + T细胞计数增加（P = 0.
