Blanco J L, Guedeja-Marrón J, Blanco I, García M E
Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
J Vet Med B Infect Dis Vet Public Health. 2000 Oct;47(8):599-605. doi: 10.1046/j.1439-0450.2000.00390.x.
Samples from otitis externa in the dog were cultured on a variety of media under different conditions of incubation in order to assess the most suitable conditions, especially for the isolation of Malassezia pachydermatis. Columbia blood and MacConkey agar at 37 degrees C for 24 h with air and Sabouraud glucose agar under microaerophilic conditions for 48 h at 37 degrees C were considered the ideal combination.
从患有外耳道炎的犬只身上采集样本，在不同培养条件下接种于多种培养基上，以评估最适宜的条件，特别是用于分离厚皮马拉色菌。认为在37℃下将样本接种于哥伦比亚血琼脂和麦康凯琼脂上并在空气中培养24小时，以及在37℃的微需氧条件下将样本接种于沙氏葡萄糖琼脂上培养48小时，是理想的组合。
