Lai M, Menichella G, Pierelli L, Serafini R, Rumi C, Sica S, Candido A, Leone G
Chair of Hematology, Università Cattolica del Sacro Cuore, Roma, Italy.
Int J Artif Organs. 2000 Oct;23(10):703-9.
This study evaluates stem cell collection procedures performed with the Dideco Excel blood cell separator, with particular attention given to yields and separator collection efficiencies. Patients' blood precounts and yield parameters related to the harvest capacity of the collection system were investigated. Fifty-five collection procedures were analyzed in 32 patients suffering from hematological malignancies and solid tumors and mobilized with chemotherapy plus G-CSF. The median blood volume processed in each procedure was 15.8 liters (12-19.750), with a blood flow rate of 70 ml/min. Patients had the following median blood precount value: NC 7.81x10(9)/L, CD34+ cells 49.08x10(3)/ml. Leukapheresis procedures gave the following yields: NC 14.95x10(9), MNC 10.83x10(9), CD34+ cells 4.37x10(6); yields/kg, NC 0.21x10(9)kg, MNC 0. 15x10(9)/kg CD34+ cells 4.26x10(6)/kg. Procedures show the following collection efficiencies: NC 10.79%, MNC 29.06%, CD34+ 42.33%, PLT 26.5%. The RBC (red blood cell) contamination of the product was (median value) 20.9 ml for each procedure, and for platelets 1.76x10(11) per procedure. The CD34+ cell precounts strongly correlated with the CD34+ yields/kg (r=0.82. p=0.000). Furthermore the NC and MNC precounts correlated with the CD34+ yields/kg but only the MNC precount correlation is notable (r=0.57, p=0.000). The logistic regression analysis shows that CD34+ (p=0.008) but not NC (po=0.14), MNC (p=0.09), or PLT (p=0.53) precounts significantly influenced the collection of a sufficient dose of CD34+ cells for transplantation (> or =2.5x10(6)/kg). Eleven of the thirty-two patients have been transplanted till now, and all had a prompt and lasting trilineage engraftment NC >1x10(9)/L on day 12 (10-17). Our data show that the collection system analyzed in this report is able to collect large amounts of progenitor cells, harvesting >2.5x10(6)/kg CD34+ cells with a single procedure in 68.8% of patients and assuring complete recovery after stem cell transplantation.
本研究评估了使用迪德科Excel血细胞分离机进行的干细胞采集程序，特别关注采集量和分离机的采集效率。研究了患者的血液预计数以及与采集系统采集能力相关的采集参数。对32例血液系统恶性肿瘤和实体瘤患者进行化疗加粒细胞集落刺激因子动员后的55次采集程序进行了分析。每次程序处理的中位血量为15.8升（12 - 19.750），血流速度为70毫升/分钟。患者的中位血液预计数如下：中性粒细胞（NC）7.81×10⁹/L，CD34⁺细胞49.08×10³/ml。白细胞单采程序的采集量如下：NC 14.95×10⁹，单核细胞（MNC）10.83×10⁹，CD34⁺细胞4.37×10⁶；每千克采集量，NC 0.21×10⁹/kg，MNC 0.15×10⁹/kg，CD34⁺细胞4.26×10⁶/kg。程序显示出以下采集效率：NC 10.79%，MNC 29.06%，CD34⁺ 42.33%，血小板（PLT）26.5%。每次程序产品中的红细胞（RBC）污染量（中位值）为20.9毫升，血小板污染量为每次程序1.76×10¹¹。CD34⁺细胞预计数与每千克CD34⁺细胞采集量密切相关（r = 0.82，p = 0.000）。此外，NC和MNC预计数与每千克CD34⁺细胞采集量相关，但只有MNC预计数的相关性显著（r = 0.57，p = 0.000）。逻辑回归分析表明，CD34⁺（p = 0.008）而非NC（p = 0.14）、MNC（p = 0.09）或PLT（p = 0.53）预计数显著影响了采集到足够剂量用于移植的CD34⁺细胞（≥2.5×10⁶/kg）。32例患者中有11例目前已接受移植，所有患者均在第12天（10 - 17天）迅速且持久地实现了三系造血重建，NC>1×10⁹/L。我们的数据表明，本报告中分析的采集系统能够采集大量祖细胞，68.8%的患者单次程序采集的CD34⁺细胞>2.5×10⁶/kg，并确保干细胞移植后完全恢复。
