Chentanez V, Chittmittrapap S, Kasantikul V
Department of Anatomy, Chulalongkorn University, Bangkok, Thailand.
J Med Assoc Thai. 2000 Sep;83(9):1101-4.
Demonstration of the increasing activity of acetylcholinesterase (AChE) in a segment of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung's disease. Two methods of histochemical assessment were tried to establish the most appropriate and effective method for this study within the limitation of available equipment. Lake's method was chosen and was modified as the standard histochemical examination.
结肠段乙酰胆碱酯酶（AChE）活性增加已被证明是诊断先天性巨结肠最准确的诊断工具。在现有设备的限制下，尝试了两种组织化学评估方法，以确定本研究最合适、最有效的方法。选择了莱克氏方法并对其进行修改，作为标准组织化学检查方法。
