“垃圾”DNA大多是内含子DNA吗？
Is "junk" DNA mostly intron DNA?
作者信息
Wong G K, Passey D A, Huang Y, Yang Z, Yu J
机构信息
Human Genome Center, Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
出版信息
Genome Res. 2000 Nov;10(11):1672-8. doi: 10.1101/gr.148900.
Abstract
Among higher eukaryotes, very little of the genome codes for protein. What is in the rest of the genome, or the "junk" DNA, that, in Homo sapiens, is estimated to be almost 97% of the genome? Is it possible that much of this "junk" is intron DNA? This is not a question that can be answered just by looking at the published data, even from the finished genomes. One cannot assume that there are no genes in a sequenced region, just because no genes were annotated. We introduce another approach to this problem, based on an analysis of the cDNA-to-genomic alignments, in all of the complete or nearly-complete genomes from the multicellular organisms. Our conclusion is that, in animals but not in plants, most of the "junk" is intron DNA.
摘要
在高等真核生物中，基因组中只有很少一部分编码蛋白质。在人类基因组中，估计约97%的基因组属于其余部分，即“垃圾”DNA，那么这些“垃圾”DNA里都有什么呢？其中大部分“垃圾”有可能是内含子DNA吗？即使参考已完成测序的基因组所发布的数据，这个问题也无法得到解答。不能仅仅因为某个测序区域未注释出基因，就认定该区域不存在基因。我们基于对多细胞生物所有完整或近乎完整基因组中cDNA与基因组比对的分析，引入了另一种解决该问题的方法。我们的结论是，在动物中，而非植物中，大部分“垃圾”是内含子DNA。
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