大肠杆菌lacZ基因内形成活性杂合β-半乳糖苷酶分子的位点。
Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.
作者信息
Brickman E, Silhavy T J, Bassford P J, Shuman H A, Beckwith J R
出版信息
J Bacteriol. 1979 Jul;139(1):13-8. doi: 10.1128/jb.139.1.13-18.1979.
Abstract
We describe the genetic analysis of 21 Escherichia coli strains in which the amino-terminal sequence of beta-galactosidase has been removed and replaced by an amino-terminal sequence from one or another of the proteins involved in maltose transport. Genetic mapping of the lacZ end of these fused genes indicates that only those fusions in which fewer than 41 amino acids are removed from the amino-terminal sequence of beta-galactosidase result in enzymatically active molecules. Within the region between amino acid 17 and amino acid 41 there are at least four or five sites where enzymatically active hybrid proteins can be formed.
摘要
我们描述了对21株大肠杆菌的遗传分析，这些菌株中β-半乳糖苷酶的氨基末端序列已被去除，并被参与麦芽糖转运的一种或另一种蛋白质的氨基末端序列所取代。这些融合基因的lacZ末端的遗传图谱表明，只有那些从β-半乳糖苷酶的氨基末端序列中去除少于41个氨基酸的融合才会产生具有酶活性的分子。在氨基酸17至氨基酸41之间的区域内，至少有四到五个位点可以形成具有酶活性的杂合蛋白。
相似文献
1
Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.
J Bacteriol. 1979 Jul;139(1):13-8. doi: 10.1128/jb.139.1.13-18.1979.
2
New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.
Gene. 1983 Nov;25(1):71-82. doi: 10.1016/0378-1119(83)90169-5.
3
Positions of early nonsense and deletion mutations in lacZ.
J Bacteriol. 1980 May;142(2):732-4. doi: 10.1128/jb.142.2.732-734.1980.
4
Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.
Methods Enzymol. 1983;101:167-80. doi: 10.1016/0076-6879(83)01012-5.
5
In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
J Bacteriol. 1980 Aug;143(2):971-80. doi: 10.1128/jb.143.2.971-980.1980.
6
Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.
J Bacteriol. 1979 Jul;139(1):19-31. doi: 10.1128/jb.139.1.19-31.1979.
7
Sequence of the lacZ gene of Escherichia coli.
EMBO J. 1983;2(4):593-7. doi: 10.1002/j.1460-2075.1983.tb01468.x.
8
Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product.
Nucleic Acids Res. 1984 Mar 12;12(5):2327-41. doi: 10.1093/nar/12.5.2327.
9
Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12.
J Bacteriol. 1986 Jan;165(1):181-92. doi: 10.1128/jb.165.1.181-192.1986.
10
Degradation of missense mutant beta-galactosidase proteins in Escherichia coli K-12.
Mol Gen Genet. 1978 Aug 4;164(1):105-8. doi: 10.1007/BF00267605.
引用本文的文献
1
CRISPR/Cas9-Mediated Genome Editing of T4 Bacteriophage for High-Throughput Antimicrobial Susceptibility Testing.
Anal Chem. 2024 Nov 12;96(45):18301-18310. doi: 10.1021/acs.analchem.4c05177. Epub 2024 Oct 30.
2
Degradation of the Escherichia coli Essential Proteins DapB and Dxr Results in Oxidative Stress, which Contributes to Lethality through Incomplete Base Excision Repair.
mBio. 2021 Feb 22;13(1):e0375621. doi: 10.1128/mbio.03756-21. Epub 2022 Feb 8.
3
Incomplete base excision repair contributes to cell death from antibiotics and other stresses.
DNA Repair (Amst). 2018 Nov;71:108-117. doi: 10.1016/j.dnarep.2018.08.014. Epub 2018 Aug 25.
4
Lethality of MalE-LacZ hybrid protein shares mechanistic attributes with oxidative component of antibiotic lethality.
Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):9164-9169. doi: 10.1073/pnas.1707466114. Epub 2017 Aug 9.
5
An estimate of the frequency of in vivo transcriptional errors at a nonsense codon in Escherichia coli.
Mol Gen Genet. 1981;183(3):561-3. doi: 10.1007/BF00268784.
6
Mutants which make more malT product, the activator of the maltose regulon in Escherichia coli.
Mol Gen Genet. 1980;178(3):589-95. doi: 10.1007/BF00337865.
7
Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2460-4. doi: 10.1073/pnas.78.4.2460.
8
Positions of early nonsense and deletion mutations in lacZ.
J Bacteriol. 1980 May;142(2):732-4. doi: 10.1128/jb.142.2.732-734.1980.
9
Export and processing of MalE-LacZ hybrid proteins in Escherichia coli.
J Bacteriol. 1984 Nov;160(2):612-7. doi: 10.1128/jb.160.2.612-617.1984.
10
In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.
Proc Natl Acad Sci U S A. 1984 Jan;81(2):535-9. doi: 10.1073/pnas.81.2.535.
本文引用的文献
1
Nonsense mutants and polarity in the lac operon of Escherichia coli.
J Mol Biol. 1965 Nov;14(1):290-6. doi: 10.1016/s0022-2836(65)80250-9.
2
Mutagens which cause deletions in Escherichia coli.
Genetics. 1969 Feb;61(2):371-6. doi: 10.1093/genetics/61.2.371.
3
Lac repressor can be fused to beta-galactosidase.
Nature. 1974 Jun 7;249(457):561-3. doi: 10.1038/249561a0.
4
Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12.
Genetics. 1974 Feb;76(2):169-84. doi: 10.1093/genetics/76.2.169.
5
Active transport of maltose in Escherichia coli K12. Involvement of a "periplasmic" maltose binding protein.
Eur J Biochem. 1974 Aug 15;47(1):139-49. doi: 10.1111/j.1432-1033.1974.tb03677.x.
6
Isolation of the bacteriophage lambda receptor from Escherichia coli.
J Bacteriol. 1973 Dec;116(3):1436-46. doi: 10.1128/jb.116.3.1436-1446.1973.
7
Molecular basis of beta-galactosidase alpha-complementation.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1254-7. doi: 10.1073/pnas.72.4.1254.
8
Conversion of beta-galactosidase to a membrane-bound state by gene fusion.
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3423-7. doi: 10.1073/pnas.73.10.3423.
9
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J Mol Biol. 1976 Jul 5;104(3):541-55. doi: 10.1016/0022-2836(76)90119-4.
10
Maltose transport in Escherichia coli K12. A comparison of transport kinetics in wild-type and lambda-resistant mutants as measured by fluorescence quenching.
Eur J Biochem. 1976 May 17;65(1):13-9. doi: 10.1111/j.1432-1033.1976.tb10383.x.
Zotero 插件