Kalnins A, Otto K, Rüther U, Müller-Hill B
EMBO J. 1983;2(4):593-7. doi: 10.1002/j.1460-2075.1983.tb01468.x.
The nucleotide sequence of the lacZ gene coding for beta-galactosidase (EC 3.2.1.23) in Escherichia coli has been determined. Beta-Galactosidase is predicted to consist of 1023 residues, resulting in a protein with a mol. wt. of 116 353 per subunit. The protein sequence originally determined by Fowler and Zabin was shown to be essentially correct and in an Appendix these authors comment on the discrepancies.
已确定大肠杆菌中编码β-半乳糖苷酶(EC 3.2.1.23)的lacZ基因的核苷酸序列。据预测,β-半乳糖苷酶由1023个残基组成,每个亚基的分子量为116353。最初由福勒和扎宾确定的蛋白质序列被证明基本正确,这些作者在附录中对差异进行了评论。