Kabir S
Academic Research and Information Management, Stockholm, Sweden.
Cytobios. 2000;103(403):121-31.
The seeds of Trichosanthes dioica contain a large amount of peptides in the range of 2-8 kD. These peptides can be resolved in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system using Tricine as the trailing ion. The seed proteins contain a number of charge species as determined by isoelectric focusing (IEF) in polyacrylamide gels. The peptides were focused in the basic region as determined by two-dimensional electrophoresis involving IEF and SDS-PAGE. The seed peptides have the unique property of being resistant to the action of silver nitrate, a sensitive reagent commonly used to stain proteins. The seed contains haemagglutinating activity which is inhibited by galactose.
栝楼种子含有大量分子量在2 - 8千道尔顿范围内的肽。这些肽可以在使用三羟甲基甘氨酸作为尾随离子的不连续十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）系统中分离。种子蛋白含有多种电荷种类，这是通过聚丙烯酰胺凝胶中的等电聚焦（IEF）确定的。通过涉及IEF和SDS - PAGE的二维电泳确定，这些肽聚焦在碱性区域。种子肽具有独特的性质，即对硝酸银（一种常用于蛋白质染色的灵敏试剂）的作用具有抗性。种子含有血凝活性，该活性受到半乳糖的抑制。
