Cornelissen A M, Maltha J C, Von den Hoff J W, Kuijpers-Jagtman A M
Department of Orthodontics and Oral Biology, College of Dental Science, University of Nijmegen, The Netherlands.
J Dent Res. 2000 Oct;79(10):1782-8. doi: 10.1177/00220345000790100901.
Wound contraction and scar formation after cleft palate surgery impair maxillary growth and dentoalveolar development. Since myofibroblast numbers and scar formation are reduced by interferon-gamma (IFN-gamma) in the healing of dermal wounds, the hypothesis was tested that local administration of IFN-gamma reduces the numbers of myofibroblasts and the elevated amount of collagen during palatal mucoperiosteal wound healing. Standardized mucoperiosteal excision wounds were made in the palatal mucoperiosteum of young rats. Either IFN-gamma or vehicle alone (sham group) was repeatedly injected into the wound site between 4 and 29 days post-wounding. The results were compared with unmanipulated control wounds. Samples of wound tissue were prepared for biochemical and microscopic analysis. The hydroxyproline, sulfated glycosaminoglycan and DNA contents of the wound tissues were analyzed biochemically. The degree of re-epithelialization, tissue thickness, the numbers of myofibroblasts, and the amounts of elastin and collagen types I and III were evaluated on histological sections. Injection of vehicle alone affected almost all healing parameters, compared with the controls, and delayed the wound-healing process. IFN-gamma stimulated re-epithelialization and decreased the numbers of myofibroblasts when compared with vehicle-treated wounds. It also decreased the hydroxyproline and glycosaminoglycan contents of 60-day-old wound tissue, but the histological characteristics of scar tissue persisted. Therefore, IFN-gamma is able to reduce the numbers of myofibroblasts and the collagen content of scar tissue after palatal wound healing. It may be a promising pharmaceutical agent for the reduction of wound contraction and scarring after cleft palate surgery.
腭裂手术后的伤口收缩和瘢痕形成会损害上颌骨生长和牙槽骨发育。由于在皮肤伤口愈合过程中，干扰素-γ（IFN-γ）可减少肌成纤维细胞数量和瘢痕形成，因此对局部应用IFN-γ能否减少腭黏骨膜伤口愈合过程中肌成纤维细胞数量及升高的胶原蛋白量这一假说进行了验证。在幼年大鼠的腭黏骨膜上制作标准化的黏骨膜切除伤口。在伤口后4至29天之间，将IFN-γ或单独的赋形剂（假手术组）反复注射到伤口部位。将结果与未处理的对照伤口进行比较。制备伤口组织样本用于生化和显微镜分析。对伤口组织的羟脯氨酸、硫酸化糖胺聚糖和DNA含量进行生化分析。在组织学切片上评估再上皮化程度、组织厚度、肌成纤维细胞数量以及弹性蛋白和I型和III型胶原蛋白的含量。与对照组相比，单独注射赋形剂几乎影响了所有愈合参数，并延迟了伤口愈合过程。与赋形剂处理的伤口相比，IFN-γ刺激了再上皮化并减少了肌成纤维细胞数量。它还降低了60日龄伤口组织的羟脯氨酸和糖胺聚糖含量，但瘢痕组织的组织学特征仍然存在。因此，IFN-γ能够减少腭伤口愈合后瘢痕组织中的肌成纤维细胞数量和胶原蛋白含量。它可能是一种有前景的药物，可减少腭裂手术后的伤口收缩和瘢痕形成。
