枯草芽孢杆菌转化过程中的基因重组：一种脱氧核糖核酸甲基化酶的出现。
Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.
作者信息
Ganesan A T
出版信息
J Bacteriol. 1979 Jul;139(1):270-9. doi: 10.1128/jb.139.1.270-279.1979.
Abstract
In Bacillus subtilis the ability to take up deoxyribonucleic acid (DNA) and undergo genetic transformation may coincide with the induction of defective phage(s) and the expression of possibly related cryptic genes. A restriction-modification enzyme system appears to be expressed. Targets of the restriction activity on the DNA can be blocked my methylation catalyzed by the methyl transferase. It is shown that cellular DNA becomes progressively methylated and reaches the maxium level during the peak of competency. Deoxycytidine residues of both incoming donor and resident DNA are methylated. The possible participation of these enzymes in recombination and the general role of cryptic genes in inducible functions are discussed.
摘要
在枯草芽孢杆菌中，摄取脱氧核糖核酸（DNA）并进行遗传转化的能力可能与缺陷噬菌体的诱导以及可能相关的隐蔽基因的表达同时发生。一种限制修饰酶系统似乎被表达出来。DNA上限制活性的靶标可以被甲基转移酶催化的甲基化作用所阻断。结果表明，细胞DNA逐渐被甲基化，并在感受态高峰期达到最高水平。进入的供体DNA和宿主DNA的脱氧胞苷残基都被甲基化。讨论了这些酶在重组中的可能参与以及隐蔽基因在诱导功能中的一般作用。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.关于肺炎球菌转化中脱氧核糖核酸整合的机制
Proc Natl Acad Sci U S A. 1964 Aug;52(2):412-9. doi: 10.1073/pnas.52.2.412.
4
BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌中脱氧核糖核酸转化早期阶段的生物合成潜伏期
J Bacteriol. 1963 Oct;86(4):785-96. doi: 10.1128/jb.86.4.785-796.1963.
5
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Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
6
Defective bacteriophages.缺陷噬菌体
J Cell Physiol. 1970 Dec;76(3):253-63. doi: 10.1002/jcp.1040760305.
7
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J Gen Physiol. 1966 Jul;49(6):197-209. doi: 10.1085/jgp.49.6.197.
8
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