Pinto M, Guerineau M, Paoletti C
Mutat Res. 1975 Nov;30(2):219-28.
The mutagenesis by ethidium bromide, an intercalating dye, which induces the mutation from wild type (rho+) to the cytoplasmic respiratory deficient petite (rho-) in Saccharomyces cerevisiae, was studied under aerobic and anaerobic conditions. During growth of anaerobic cells at pH 6.5, ethidium bromide at a concentration of 2 mug/ml is unable to induce rho- mutants whereas under aerobic conditions the entire population is converted into rho- cells within 1 generation at the same drug concentration. With ethidium bromide 10 mug/ml 98% of the anaerobic cells are transformed into rho- in 5.5 h (more than 2 generations). In non-growing conditions, ethidium bromide 10 mug/ml has no effect in anaerobic cells. 3 h adapted cells used as control, are converted into rho- in 8 h. Increasing the ethidium bromide concentration to 20 mug/ml resulted in the appearance of some rho- mutants in the anaerobic population but marked at the same time the onset of a detectable toxic effect of the drug.
研究了在需氧和厌氧条件下，嵌入染料溴化乙锭对酿酒酵母的诱变作用，该染料可诱导野生型（rho +）突变为细胞质呼吸缺陷型小菌落（rho -）。在pH 6.5的厌氧细胞生长过程中，浓度为2μg/ml的溴化乙锭无法诱导rho -突变体，而在需氧条件下，相同药物浓度下整个群体在1代内就会转变为rho -细胞。使用10μg/ml的溴化乙锭时，98%的厌氧细胞在5.5小时内（超过2代）转变为rho -。在非生长条件下，10μg/ml的溴化乙锭对厌氧细胞没有影响。用作对照的适应3小时的细胞，在8小时内转变为rho -。将溴化乙锭浓度提高到20μg/ml会导致厌氧群体中出现一些rho -突变体，但同时也标志着该药物可检测到的毒性作用开始出现。
