Alcántara C, Cervera J, Rubio V
Instituto de Investigaciones Citológicas (FVIB), Valencia, Spain.
FEBS Lett. 2000 Nov 10;484(3):261-4. doi: 10.1016/s0014-5793(00)02168-2.
The exclusive involvement of carbamate kinase (CK) in fermentative ATP production and of carbamoyl phosphate synthetase (CPS) in the production of carbamoyl phosphate (CP) for pyrimidines and arginine biosynthesis was challenged by the finding of CK as the only activity synthesising CP in the archaea Pyrococcus furiosus and Pyrococcus abyssi. We now show that CK can replace CPS in vivo: transformation of Escherichia coli devoid of the CPS gene with plasmids encoding the CK from P. furiosus or from Enterococcus faecalis (which uses CK for making ATP) restores the ability of CPS-deficient E. coli to grow in the absence of arginine and uracil if ammonia and bicarbonate are present.
在激烈火球菌（Pyrococcus furiosus）和深渊火球菌（Pyrococcus abyssi）古菌中，氨基甲酸激酶（CK）是合成氨基甲酰磷酸（CP）的唯一活性物质，这一发现对CK仅参与发酵性ATP生成以及氨甲酰磷酸合成酶（CPS）仅参与嘧啶和精氨酸生物合成所需的CP生成这一观点提出了挑战。我们现在表明，CK在体内可替代CPS：用编码来自激烈火球菌或粪肠球菌（利用CK生成ATP）的CK的质粒转化缺乏CPS基因的大肠杆菌，如果存在氨和碳酸氢盐，可恢复CPS缺陷型大肠杆菌在无精氨酸和尿嘧啶情况下生长的能力。
