人瘦素的体外分泌具有性别依赖性，但与供体的体重指数无关。

The in vitro secretion of human leptin is gender-dependent but independent of the body mass index of the donors.

作者信息

Menendez C, Baldelli R, Lage M, Casabiell X, Piñero V, Solar J, Dieguez C, Casanueva F F

机构信息

Department of Medicine, Molecular Endocrinology Section, Santiago de Compostela University, Spain.

出版信息

Eur J Endocrinol. 2000 Nov;143(5):711-4. doi: 10.1530/eje.0.1430711.

DOI:10.1530/eje.0.1430711

PMID:11078997

Abstract

OBJECTIVE

Leptin is an adipocyte-secreted hormone acting as a signal to the central nervous system, where it regulates energy homeostasis and neuroendocrine processes. Leptin plasma levels are mainly regulated by the percentage of body fat, but are also controlled by several metabolic and nutritional variables. Data regarding leptin secretion suggest that it is gender regulated, and higher levels are present in women than men; however, the biological basis for this sex-related difference is unknown. To clarify those points, a systematic study with tissue cultures from human omental adipose tissue was performed.

DESIGN AND METHODS

Surgically obtained samples from 137 patients (68 women, 69 men) were evaluated. The assay was standardized in periods of 24 h ending at 96 h. Each adipose tissue sample from a single donor was incubated in triplicate and leptin results expressed as the mean of the integrated secretion into the medium (nanograms of leptin/g tissue per time).

RESULTS

Tissue adipose cultures showed a steady leptin secretion throughout the 96 h studied, with the peak of secretory activity reached at 48 h; afterwards, the in vitro secretion reached a plateau state. Spontaneous leptin secretion in the 24 h and 48 h period, as well as the area under the curve analyzed in the 0-48 h period, showed a gender-based difference that was significantly (P<0. 05) higher in women than in men. When data of spontaneous leptin secretion were correlated with the body mass index (BMI) of the donors, no correlation was found. This suggests that in vivo leptin levels are dependent on the total amount of fat of the individual, but independent of the leptin secretory rate by the adipose tissue of the donor.

CONCLUSIONS

Leptin secretion from omental adipose tissue in vitro is: (i) significantly higher in samples from women than in samples from men; and (ii) not correlated with the BMI, showing that in vitro leptin secretion is not related to the adiposity of the donor.

摘要

目的

瘦素是一种由脂肪细胞分泌的激素，作为一种信号作用于中枢神经系统，在中枢神经系统中调节能量平衡和神经内分泌过程。瘦素的血浆水平主要受体脂百分比调节，但也受多种代谢和营养变量的控制。关于瘦素分泌的数据表明其分泌存在性别差异，女性的瘦素水平高于男性；然而，这种性别相关差异的生物学基础尚不清楚。为了阐明这些问题，我们对人网膜脂肪组织的组织培养进行了系统研究。

设计与方法

对137例患者（68例女性，69例男性）手术获取的样本进行评估。检测在以96小时结束的24小时时间段内进行标准化。来自单个供体的每个脂肪组织样本一式三份进行培养，瘦素结果表示为培养基中综合分泌的平均值（每单位时间每克组织中瘦素的纳克数）。

结果

在整个96小时的研究过程中，组织脂肪培养显示瘦素分泌稳定，分泌活性在48小时达到峰值；之后，体外分泌达到平台期。24小时和48小时期间的自发瘦素分泌，以及0 - 48小时期间分析的曲线下面积，显示出性别差异，女性显著高于男性（P<0.05）。当自发瘦素分泌数据与供体的体重指数（BMI）相关联时，则未发现相关性。这表明体内瘦素水平取决于个体的脂肪总量，但与供体脂肪组织的瘦素分泌率无关。