Fokin A A, Robicsek F, Masters T N
Department of Thoracic and Cardiovascular Surgery, Heineman Medical Research Laboratory, Carolinas Medical Center, Charlotte, NC 28203, USA.
Microcirculation. 2000 Oct;7(5):357-65.
Investigation of the transport of viral-size particles after intravenous versus intralymphatic injection and the functional validity of lymphatico-venous communications.
In the canine model, [99mTc] sulfur colloid particles (100-200 nm) were injected into either the principal vein or into the main lymphatic channel exposed at the paw. Samples of blood and lymph were collected at the groin from the cannulated femoral vein and from a major lymphatic vessel. Parameters including particle arrival time, concentration, flux, and accumulation were determined for a 45-minute period using gamma counting.
After intralymphatic injection, particles arrived in the venous blood in an average of 4 seconds. The mean arrival time of particles in the lymph after intravenous injection was 25.4 +/- 6.44 minutes. Intralymphatic injection increased lymph flow and enhanced particle transport. Concentration values in the venous blood after intralymphatic injection and in lymph after intravenous injection were comparable. Flux values depended primarily on flow conditions. Particle accumulation in the lymph after intravenous injection was delayed, but continued to increase throughout the experiment.
There are functional lymphatico-venous communications at the very peripheral level under physiological conditions, which allow rapid transport of viral-size particulate matter between the two pathways and may contribute to the spread of viral infection.
研究静脉注射与淋巴管内注射后病毒大小颗粒的转运情况以及淋巴管 - 静脉交通的功能有效性。
在犬模型中，将[99mTc]硫胶体颗粒（100 - 200纳米）注入主静脉或暴露于爪部的主要淋巴管。从插管的股静脉和一条主要淋巴管在腹股沟处采集血液和淋巴样本。使用γ计数在45分钟内测定包括颗粒到达时间、浓度、通量和蓄积等参数。
淋巴管内注射后，颗粒平均在4秒内进入静脉血。静脉注射后颗粒在淋巴中的平均到达时间为25.4 +/- 6.44分钟。淋巴管内注射增加了淋巴流量并增强了颗粒转运。淋巴管内注射后静脉血中的浓度值与静脉注射后淋巴中的浓度值相当。通量值主要取决于流动条件。静脉注射后颗粒在淋巴中的蓄积延迟，但在整个实验过程中持续增加。
在生理条件下，非常外周水平存在功能性淋巴管 - 静脉交通，这使得病毒大小颗粒物质能够在两条途径之间快速转运，并可能有助于病毒感染的传播。
