谷胱甘肽过氧化物酶和过氧化氢酶对光氧化补骨脂素诱导的溶血和高铁血红蛋白修饰的影响。
Effects of glutathione peroxidase and catalase on hemolysis and methemoglobin modifications induced by photooxidized psoralen.
作者信息
Lysenko E P, Melnikova V O, Andina E S, Wunderlich S, Pliquett F, Potapenko A Y
机构信息
Department of Medical and Biological Physics, Russian State Medical University, Moscow.
出版信息
J Photochem Photobiol B. 2000 Jul;56(2-3):187-95. doi: 10.1016/s1011-1344(00)00079-8.
Psoralen photooxidation products (POP products) were obtained by UVA irradiation (365 nm, 180-640 W/m2) of an aqueous psoralen solution with fluences of 0-800 kJ/m2. Preincubation of POP products with glutathione peroxidase (GSHPer) or catalase, as well as presence of catalase during UVA irradiation of the aqueous psoralen solution did not influence their hemolytic activity. However, both GSHPer and catalase inhibited POP-induced conversion of methemoglobin. This indicates that hydrogen peroxide and psoralen peroxides destructible by GSHPer, which are being produced during psoralen photooxidation, do not possess hemolytic activity. Furthermore, hydrogen peroxide does not appear to serve as an intermediate in the process of hemolysin formation. Hydrogen peroxide generated during psoralen photooxidation is apparently the main POP product responsible for MetHb conversion.
补骨脂素光氧化产物（POP产物）是通过用UVA（365纳米，180 - 640瓦/平方米）照射补骨脂素水溶液（通量为0 - 800千焦/平方米）获得的。将POP产物与谷胱甘肽过氧化物酶（GSHPer）或过氧化氢酶预孵育，以及在补骨脂素水溶液的UVA照射过程中存在过氧化氢酶，均不影响它们的溶血活性。然而，GSHPer和过氧化氢酶均抑制POP诱导的高铁血红蛋白转化。这表明在补骨脂素光氧化过程中产生的、可被GSHPer破坏的过氧化氢和补骨脂素过氧化物不具有溶血活性。此外，过氧化氢似乎不是溶血素形成过程中的中间体。补骨脂素光氧化过程中产生的过氧化氢显然是负责高铁血红蛋白转化的主要POP产物。
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