Saparov S M, Agamalieva M A, Lysenko E P, Bezdetnaya L N, Sukhorukov V L
Department of Medical and Biological Physics, Russian State Medical University, Moscow.
J Photochem Photobiol B. 1993 Jan;17(1):69-75. doi: 10.1016/1011-1344(93)85009-w.
The influence of Fe2+ ions and reduced glutathione (GSH) on the haemolysis of erythrocytes photosensitized (366 nm) by psoralen (PUVA haemolysis) was investigated. PUVA haemolysis was induced by the low fluence rate (24 W m-2) and high fluence (greater than 150 W m-2) of UV-A irradiation. It has been shown that Fe2+ ions and GSH activated PUVA haemolysis at both fluence rates of irradiation. PUVA haemolysis activation by Fe2+ ions was more pronounced than that by GSH. It is supposed that activation caused by Fe2+ ions and GSH is connected with their ability to reduce lipid peroxides or psoralen peroxides with the subsequent formation of free radicals. The regeneration of endogenous Fe2+ by reduced glutathione is also possible.
