大肠杆菌中延伸因子Tu的含量
Content of elongation factor Tu in Escherichia coli.
作者信息
Furano A V
出版信息
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4780-4. doi: 10.1073/pnas.72.12.4780.
Abstract
The content of elongation factor Tu in E. coli B has been determined both by radioimmune assay and by GDP binding. The two assays gave comparable results: cells growing at 2 doublings per hour contained about 8 molecules of Tu per ribosome, whereas those growing at 0.22 doublings per hour contained about 14 molecules per ribosome. These levels resemble those reported for tRNA, in contrast with the 1:1 ratio of factor to ribosomes reported for elongation factors Ts and G.
摘要
通过放射免疫测定法和GDP结合法测定了大肠杆菌B中延伸因子Tu的含量。这两种测定方法得出了可比的结果：每小时倍增2次生长的细胞，每个核糖体约含8个Tu分子，而每小时倍增0.22次生长的细胞，每个核糖体约含14个分子。这些水平与报道的tRNA水平相似，这与报道的延伸因子Ts和G与核糖体1:1的比例形成对比。
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