Solter D, Knowles B B
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5099-102. doi: 10.1073/pnas.72.12.5099.
Mouse blastocysts with and without zonae pellucidae are susceptible to complement-dependent antibody cytotoxicity. Exposure of blastocysts to rabbit anti-mouse serum together with complement results in the death of all cells; however, when blastocysts are exposed to antiserum alone and then transferred to guinea pig complement, only the trophoblastic cells are killed. These results suggest that the mouse blastocyst is not permeable for certain antibodies. The inner cell masses can easily be separated from the remnants of trophoblastic cells and are then able to grow and differentiate in vitro. This method of immunosurgery can be used to obtain large quantities of pure inner cell masses in a relatively short period of time.
有透明带和无透明带的小鼠囊胚对补体依赖性抗体细胞毒性敏感。将囊胚与兔抗小鼠血清及补体一起孵育会导致所有细胞死亡；然而，当囊胚单独与抗血清孵育后再转移至豚鼠补体中时，只有滋养层细胞会被杀死。这些结果表明，小鼠囊胚对某些抗体是不可渗透的。内细胞团能够轻易地与滋养层细胞残余物分离，然后能够在体外生长和分化。这种免疫手术方法可用于在相对较短的时间内获得大量纯净的内细胞团。
Zotero 插件