Dube S, Dolcini G, Abbott L, Mehta S, Dube D, Gutierrez S, Ceriani C, Esteban E, Ferrer J, Poiesz B
Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA.
Virology. 2000 Nov 25;277(2):379-86. doi: 10.1006/viro.2000.0622.
DNA was extracted from the peripheral blood of a seropositive, PCR-positive, BLV-infected Holstein cow (No. 38) from Argentina. The DNA was amplified via PCR with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 38 DNA was cloned, sequenced, and compared phylogenetically to three other full-length BLV sequences. Characterization of its deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
从一头来自阿根廷的血清学阳性、PCR阳性、感染牛白血病病毒（BLV）的荷斯坦奶牛（编号38）的外周血中提取DNA。使用一系列覆盖整个BLV前病毒DNA的重叠引物通过PCR扩增该DNA。扩增得到的BLV ARG 38 DNA被克隆、测序，并与其他三个全长BLV序列进行系统发育比较。文中还讨论了其推导蛋白的特征以及它与逆转录病毒PTLV/BLV属其他成员的关系。
