Kuczyński W, Pietrewicz P, Grygoruk C, Redźko S, Grochowski D, Szamatowicz J, Wasilewski T, Wołczyński S, Syrewicz M, Domitrz J, Szamatowicz M
Kliniki Ginekologii AM w Białymstoku.
Ginekol Pol. 2000 Sep;71(9):1053-8.
To analyze the influence of intracytoplasmic sperm injection (ICSI) on early embryo development used for reinsemination of unfertilized 1-day-old oocytes.
Prospective observational study.
A total of 126 embryos resulted from reinsemination by ICSI were analyzed with regard for the time course of cleavage and the quality of embryos at 48 hours after injection. Results were compared with those obtained in 698 embryos obtained after routine ICSI program.
It has been shown that embryos derived from reinsemination developed slower in comparison with those obtained after regular ICSI program. At 48 hours after microinjection 50% of embryos achieved 4 blastomeres stage and 37% remained on 2 cells stage. 71% of regular ICSI embryos at this same time showed 4 blastomere or more, only 16% remained on 2 blastomeres stage. The quality of embryos was similar in two compared groups, however those obtained following reinsemination at 4 blastomere stage were significantly poorer quality.
Embryos derived from reinsemination of 1-day old oocytes unfertilized after conventional IVF developed slower and the quality of embryos at 4 blastomere stage was poorer comparing to regular ICSI embryos.
分析卵胞浆内单精子注射（ICSI）对用于未受精1日龄卵母细胞再受精的早期胚胎发育的影响。
前瞻性观察研究。
对通过ICSI再受精产生的126个胚胎进行分析，观察其卵裂的时间进程以及注射后48小时的胚胎质量。将结果与常规ICSI程序后获得的698个胚胎的结果进行比较。
结果显示，与常规ICSI程序后获得的胚胎相比，再受精产生的胚胎发育较慢。显微注射后48小时，50%的胚胎达到4细胞阶段，37%仍处于2细胞阶段。同期常规ICSI胚胎中71%显示为4细胞或更多细胞，只有16%仍处于2细胞阶段。两组胚胎的质量相似，但再受精后在4细胞阶段获得的胚胎质量明显较差。
与常规ICSI胚胎相比，常规体外受精后未受精的1日龄卵母细胞再受精产生的胚胎发育较慢，且4细胞阶段的胚胎质量较差。
