Morton P C, Yoder C S, Tucker M J, Wright G, Brockman W D, Kort H I
Reproductive Biology Associates, Atlanta, Georgia 30342, USA.
Fertil Steril. 1997 Sep;68(3):488-91. doi: 10.1016/s0015-0282(97)00223-9.
To analyze the efficacy of intracytoplasmic sperm injection (ICSI) for "rescue" of failed conventional insemination cycles.
Retrospective clinical study.
Private infertility clinic.
PATIENT(S): Fifty-four couples with non-male factor infertility undergoing routine IVF within our clinic.
INTERVENTION(S): Twenty- to 24-hour-old unfertilized mature oocytes from conventional IVF cycles in which fertilization failure was complete were reinseminated by ICSI to attempt late fertilization and rescue of the otherwise failed IVF cycle.
MAIN OUTCOME MEASURE(S): Late fertilization, pregnancy, and embryonic implantation.
RESULT(S): Intracytoplasmic sperm injection reinsemination of 489 unfertilized mature oocytes caused degeneration in 50 (10.2%) oocytes, gave rise to normal fertilization in 215 (44.0%) of the injected oocytes, and allowed ET in 48 of the 54 cycles in which initial complete fertilization failure had occurred. One hundred sixty-four (76.3%) of 215 late-fertilized oocytes either were used for fresh transfer or were frozen-stored. Eight viable pregnancies resulted, yielding a 14.8% pregnancy rate per initiated cycle.
CONCLUSION(S): Barring potential concerns regarding the chromosomal normality of embryos arising from reinsemination, our results suggest that ICSI is a relatively successful means of rescuing conventional IVF cycles in which fertilization fails completely.
分析卵胞浆内单精子注射（ICSI）对常规授精周期失败的“挽救”效果。
回顾性临床研究。
私立不孕不育诊所。
在我们诊所接受常规体外受精（IVF）的54对非男性因素不孕夫妇。
将常规IVF周期中20至24小时龄未受精的成熟卵母细胞（其中受精完全失败）通过ICSI重新授精，以尝试延迟受精并挽救原本失败的IVF周期。
延迟受精、妊娠和胚胎着床。
对489个未受精的成熟卵母细胞进行ICSI重新授精，导致50个（10.2%）卵母细胞退化，215个（44.0%）注射的卵母细胞实现正常受精，在最初完全受精失败的54个周期中有48个周期进行了胚胎移植（ET）。215个延迟受精的卵母细胞中有164个（76.3%）用于新鲜移植或冷冻保存。获得了8例活产妊娠，每个启动周期的妊娠率为14.8%。
除了对重新授精产生的胚胎染色体正常性存在潜在担忧外，我们的结果表明，ICSI是挽救完全受精失败的常规IVF周期的一种相对成功的方法。
