García-Sáenz M C, Arias-Puente A, Fresnadillo-Martinez M J, Matilla-Rodriguez A
Fundación Hospital de Alcorcón, Madrid, Spain.
J Cataract Refract Surg. 2000 Nov;26(11):1673-9. doi: 10.1016/s0886-3350(00)00483-1.
To compare the in vitro adherence of slime-producing and non-slime-producing Staphylococcus epidermidis to different intraocular lenses (IOLs) to study the organism's contribution to postoperative endophthalmitis.
Strains of slime-positive (ATCC 35984) and slime-negative (ATCC 12228) S epidermidis were used. The IOLs were made of poly(methyl methacrylate) (PMMA), PMMA with polypropylene haptics, silicone, hydrogel, acrylic, heparin-surface-modified (HSM) PMMA, and fluorine-surface-modified PMMA. The lenses were incubated overnight with bacteria, then sonicated and vortexed to separate the adhered bacteria. Quantitative cultures were performed and the results statistically analyzed.
Slime-negative strains of S epidermidis adhered to all IOLs but at a lower level than slime-positive strains. The most adherent lenses were acrylic with the positive strain and PMMA with the negative strain. The least adherent IOLs were PMMA with the positive strain and hydrogel with the negative strain. There were no significant differences between rigid and foldable lenses. Polypropylene was significantly more adherent than PMMA to the slime-positive strain. The acrylic and the HSM PMMA IOLs were significantly more adherent to the positive strain.
In vitro, there were significant differences in bacterial adhesion among IOL materials. Slime-positive strains of S epidermidis were more adherent than slime-negative ones.
比较产黏液和不产黏液的表皮葡萄球菌在体外对不同人工晶状体（IOL）的黏附情况，以研究该微生物在术后眼内炎中的作用。
使用黏液阳性（ATCC 35984）和黏液阴性（ATCC 12228）的表皮葡萄球菌菌株。IOL由聚甲基丙烯酸甲酯（PMMA）、带聚丙烯触觉的PMMA、硅酮、水凝胶、丙烯酸酯、肝素表面改性（HSM）的PMMA和氟表面改性的PMMA制成。将晶状体与细菌一起孵育过夜，然后进行超声处理和涡旋以分离黏附的细菌。进行定量培养并对结果进行统计分析。
黏液阴性的表皮葡萄球菌菌株可黏附于所有IOL，但黏附水平低于黏液阳性菌株。黏附性最强的晶状体是阳性菌株对应的丙烯酸酯类和阴性菌株对应的PMMA。黏附性最弱的IOL是阳性菌株对应的PMMA和阴性菌株对应的水凝胶。硬性和可折叠晶状体之间无显著差异。聚丙烯对黏液阳性菌株的黏附性明显高于PMMA。丙烯酸酯类和HSM PMMA IOL对阳性菌株的黏附性明显更高。
在体外，IOL材料之间的细菌黏附存在显著差异。黏液阳性的表皮葡萄球菌菌株比黏液阴性菌株的黏附性更强。
